Tested Applications
| Positive WB detected in | K-562 cells, HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
13406-1-AP targets ATG10 in WB, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag4052 Product name: Recombinant human ATG10 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-220 aa of BC029268 Sequence: MEEDEFIGEKTFQRYCAEFIKHSQQIGDSWEWRPSKDCSDGYMCKIHFQIKNGSVMSHLGASTHGQTCLPMEEAFELPLDDCEVIETAAASEVIKYEYHVLYSCSYQVPVLYFRASFLDGRPLTLKDIWEGVHECYKMRLLQGPWDTITQQEHPILGQPFFVLHPCKTNEFMTPVLKNSQKINKNVNYITSWLSIVGPVVGLNLPLSYAKATSQDERNVP Predict reactive species |
| Full Name | ATG10 autophagy related 10 homolog (S. cerevisiae) |
| Calculated Molecular Weight | 220 aa, 25 kDa |
| Observed Molecular Weight | 25-30 kDa |
| GenBank Accession Number | BC029268 |
| Gene Symbol | ATG10 |
| Gene ID (NCBI) | 83734 |
| RRID | AB_3669161 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9H0Y0 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
ATG10, also named as APG10L, is an E2-like enzyme involved in autophagy. It catalyzes the conjugation reaction between Atg12 and Atg5. ATG10 plays a role in adenovirus-mediated cell lysis.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for ATG10 antibody 13406-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The ATG10 Polyclonal antibody (Cat# 13406-1-AP) has 1 total citation. It appears in PLoS One (2024), in a study investigating ATRA-induced NEAT1 upregulation promoting autophagy during acute promyelocytic leukemia (APL) cell granulocytic differentiation. The research field is autophagy and hematopoietic cell differentiation, with the antibody used for Western blot in human samples.
| Species | Application | Title |
|---|---|---|
PLoS One ATRA-induced NEAT1 upregulation promotes autophagy during APL cell granulocytic differentiation |
