Tested Applications
| Positive WB detected in | Daudi cells, A549 cells, HepG2 cells, mouse lung tissue, HeLa cells |
| Positive IF/ICC detected in | HepG2 cells, Hela cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:10-1:100 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
13550-1-AP targets SFI1 in WB, IF/ICC, CoIP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag4268 Product name: Recombinant human SFI1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 917-1211 aa of BC033613 Sequence: MAQAHPSLLADLGLAFPDFQSPYKIGDPTEISDPPAAPGQPRGTDPMFLSSNTAHSARKQPRRPHFLLEPAQSQRPQKPQEHGLGMAQPAAPSLTRPFLAEAPTALVPHSPLPGALSSAPGPKQPPTASTGPELLLLPLSSFMPCGAAAPARVSAQRATPRDKPPVPSSLASVPDPHLLLPGDFSATRAGPGLSTAGSLDLEAELEEIQQQLLHYQTTKQNLWSCRRQASSLRRWLELNREEPGPEDQEVEQQVQKELEQVEMQIQLLAEELQAQRQPIGACVARIQALRQALC Predict reactive species |
| Full Name | Sfi1 homolog, spindle assembly associated (yeast) |
| Calculated Molecular Weight | 1211 aa, 134 kDa |
| Observed Molecular Weight | 148 kDa |
| GenBank Accession Number | BC033613 |
| Gene Symbol | SFI1 |
| Gene ID (NCBI) | 9814 |
| RRID | AB_10596938 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | A8K8P3 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
human SFI1 is a centriolar protein that associates with a pool of Centrin at the distal end of the centriole. SFI1 forms an interdependent complex with centrin at the centriolar distal end. Human SFI1 is an evolutionarily conserved protein of 1,242 amino acids that contains five residues within full Cdk consensus motifs (S/T*-P-x-K/R), four at the C terminus known to be phosphorylated in vivo (T816, S855, S882, S892) and one immediately preceding the C terminus (PMID: 25340401, 31197030).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for SFI1 antibody 13550-1-AP | Download protocol |
| WB protocol for SFI1 antibody 13550-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The SFI1 Polyclonal antibody (Cat# 13550-1-AP) has 10 citations published in journals including Cell, Science Advances, EBioMedicine, EMBO Journal, Science Signaling, Journal of Cell Biology, Scientific Reports, Journal of Cell Science, and bioRxiv. Research fields span centriole assembly and architecture, ciliogenesis, centrosome error-induced senescence, mitotic regulation, nucleotide excision repair, and neurodevelopment. Applications cited include IF, WB, and CoIP in human and mouse samples.
| Species | Application | Title |
|---|---|---|
Cell Time-series reconstruction of the molecular architecture of human centriole assembly | ||
Sci Adv Centrin-POC5 inner scaffold provides distal centriole integrity for sperm flagellar assembly. | ||
EBioMedicine PARP inhibitors suppress tumours via centrosome error-induced senescence independent of DNA damage response | ||
EMBO J Human SFI1 and Centrin form a complex critical for centriole architecture and ciliogenesis
| ||
Sci Signal Aurora B opposes PP1 function in mitosis by phosphorylating the conserved PP1-binding RVxF motif in PP1 regulatory proteins. | ||
J Cell Biol CCDC15 localizes to the centriole inner scaffold and controls centriole length and integrity |
