Tested Applications
| Positive WB detected in | COLO 320 cells, MCF-7 cells |
| Positive IP detected in | COLO 320 cells |
| Positive IHC detected in | human gliomas tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | MCF-7 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
13553-1-AP targets PES1 in WB, IHC, IF/ICC, IP, ChIP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse, rat, goat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag4342 Product name: Recombinant human PES1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 238-588 aa of BC032489 Sequence: LYQLLNLHYPPKLEGQAQAEAKAGEGTYALDSESCMEKLAALSASLARVVVPATEEEAEVDEFPTDGEMSAQEEDRRKELEAQEKHKKLFEGLKFFLNREVPREALAFIIRSFGGEVSWDKSLCIGATYDVTDSRITHQIVDRPGQQTSVIGRCYVQPQWVFDSVNARLLLPVAEYFSGVQLPPHLSPFVTEKEGDYVPPEKLKLLALQRGEDPGNLNESEEEEEEDDNNEGDGDEEGENEEEEEDAEAGSEKEEEARLAALEEQRMEGKKPRVMAGTLKLEDKQRLAQEEESEAKRLAIMMMKKREKYLYQKIMFGKRRKIREANKLAEKRKAHDEAVRSEKKAKKARPE Predict reactive species |
| Full Name | pescadillo homolog 1, containing BRCT domain (zebrafish) |
| Calculated Molecular Weight | 588 aa, 68 kDa |
| Observed Molecular Weight | 72 kDa |
| GenBank Accession Number | BC032489 |
| Gene Symbol | PES1 |
| Gene ID (NCBI) | 23481 |
| RRID | AB_2161967 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O00541 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for PES1 antibody 13553-1-AP | Download protocol |
| IHC protocol for PES1 antibody 13553-1-AP | Download protocol |
| IP protocol for PES1 antibody 13553-1-AP | Download protocol |
| WB protocol for PES1 antibody 13553-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The PES1 antibody (Cat# 13553-1-AP) has 14 citations across 13 journals, including Nature Chemical Biology, Journal of Experimental & Clinical Cancer Research, Advanced Science, Cell Reports, and Molecular Medicine. Associated research fields span oncology (colorectal, melanoma, hepatocellular, breast, and head and neck cancers), diabetic retinopathy, lipid metabolism, autophagy, protein kinase signaling, organelle biology, and reproductive biology.
| Species | Application | Title |
|---|---|---|
Nat Chem Biol Molecular recording of cellular protein kinase activity with chemical labeling
| ||
J Exp Clin Cancer Res SNHG17 promotes colorectal tumorigenesis and metastasis via regulating Trim23-PES1 axis and miR-339-5p-FOSL2-SNHG17 positive feedback loop. | ||
Adv Sci (Weinh) Targeting WDR12 Unleashes T-Cell-Mediated Antitumor Activity in Melanoma by Destabilizing CD276. | ||
J Transl Med ELF3-regulated PES1 targets VEGFR2 to mediate angiogenesis and retinal inner barrier injury in diabetic retinopathy.
| ||
Mol Med Ketogenic diet ameliorates lipid dysregulation in type 2 diabetic mice by downregulating hepatic pescadillo 1. | ||
Cell Rep Lactylation-stabilized NOL6 promotes colorectal cancer progression via STAMBP-mediated YY1 deubiquitination and c-Myc transcription upregulation. |
