Tested Applications
| Positive WB detected in | HEK-293 cells, Jurkat cells, mouse brain tissue, mouse thymus tissue, HeLa cells, K-562 cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human colon tissue, human colon cancer tissue, human gliomas tissue, human liver tissue, mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse brain tissue |
| Positive IF/ICC detected in | HeLa cells, HepG2 cells |
| Positive FC (Intra) detected in | Jurkat cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:12000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
13755-1-AP targets FMRP in WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag4697 Product name: Recombinant human FMR1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-297 aa of BC038998 Sequence: MEELVVEVRGSNGAFYKAFVKDVHEDSITVAFENNWQPDRQIPFHDVRFPPPVGYNKDINESDEVEVYSRANEKEPCCWWLAKVRMIKGEFYVIEYAACDATYNEIVTIERLRSVNPNKPATKDTFHKIKLDVPEDLRQMCAKEAAHKDFKKAVGAFSVTYDPENYQLVILSINEVTSKRAHMLIDMHFRSLRTKLSLIMRNEEASKQLESSRQLASRFHEQFIVREDLMGLAIGTHGANIQQARKVPGVTAIDLDEDTCTFHIYGEDQDAVKKARSFLEFAEDVIQVPRNLVGLKI Predict reactive species |
| Full Name | fragile X mental retardation 1 |
| Calculated Molecular Weight | 632 aa, 71 kDa |
| Observed Molecular Weight | 59-72 kDa |
| GenBank Accession Number | BC038998 |
| Gene Symbol | FMR1 |
| Gene ID (NCBI) | 2332 |
| RRID | AB_2262872 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q06787 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
The selective RNA-binding protein FMRP forms a messenger ribonucleoprotein complex that associates with polyribosomes, implicating in regulation of translation. FMR1 is a component of the CYFIP1-EIF4E-FMR1 complex which binds to and reperss the mRNA. It also has a role in the transport of mRNA from the nucleus to the cytoplasm. FMR1 exists several isforms and the molecular weight of FMR1 is about 59-72 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for FMRP antibody 13755-1-AP | Download protocol |
| IF protocol for FMRP antibody 13755-1-AP | Download protocol |
| IHC protocol for FMRP antibody 13755-1-AP | Download protocol |
| IP protocol for FMRP antibody 13755-1-AP | Download protocol |
| WB protocol for FMRP antibody 13755-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The FMRP Polyclonal antibody (Cat# 13755-1-AP) has 19 citations spanning journals including Cell, Cell Metabolism, Nature Communications, Advanced Science, Cell Death & Disease, Developmental Cell, Cell Reports, FASEB Journal, and PLoS One. Research fields encompass m⁶A RNA modification, cancer biology (renal, colorectal, prostate, breast, gastric, esophageal, and hepatocellular carcinomas), fragile X syndrome, stress granule dynamics, mitochondrial dysfunction, neurodegeneration, meiosis, and inflammatory signaling.
| Species | Application | Title |
|---|---|---|
Cell Competing Protein-RNA Interaction Networks Control Multiphase Intracellular Organization. | ||
Cell Metab Mitochondrial dysfunction reveals H(2)S-mediated synaptic sulfhydration as a potential mechanism for autism-associated social defects. | ||
Nat Commun Stalled translation by mitochondrial stress upregulates a CNOT4-ZNF598 ribosomal quality control pathway important for tissue homeostasis | ||
Adv Sci (Weinh) ERβ/circAHNAK Axis Inhibits USP10-FMR1 Deubiquitination to Prevent m⁶A-Mediated ADAM17 Decay and Promote Angiogenesis in Clear Cell Renal Cell Carcinoma. | ||
Adv Sci (Weinh) m6A Reader hnRNPA2B1 Modulates Late Pachytene Progression in Male Meiosis Through Post-Transcriptional Control | ||
Cell Death Dis FMR1 promotes the progression of colorectal cancer cell by stabilizing EGFR mRNA in an m6A-dependent manner
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Reviews
What customers say
Both reviewers used the antibody for Western blot at 1:1000 dilution. One user (4/5) found it sensitive enough to detect the target band in IP pull-downs, though it showed multiple bands possibly representing protein isoforms, limiting specificity. The other user (2/5) reported insufficient specificity in WB on mouse and rat brain tissue, noting the need for long exposure with highly sensitive ECL and that it performed worse than an alternative antibody they had available. Overall, sensitivity was acknowledged, but specificity concerns were the main recurring theme.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Parisa (Verified Customer) (09-26-2023) | We used for WB, nor high specificity as we could see different bands (they could be different isoforms of the protein). But it was sensitive and we could see the band pulled down in IP for our protein of interest.
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Marta (Verified Customer) (02-24-2020) | not specific enough in WB. Not tested in IPworst than the antibody that we have available in the antibody. Need a long exposure with a high sensible ECL.
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