Tested Applications
| Positive WB detected in | HeLa cells, RAW 264.7 cells, HepG2 cells, A549 cells, NIH/3T3 cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive FC (Intra) detected in | HEK-293T cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
14063-1-AP targets SETD8 in WB, IHC, IF, IP, CoIP, ChIP, ELISA, Cell treatment applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag5114 Product name: Recombinant human SETD8 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-352 aa of BC050346 Sequence: MARGRKMSKPRAVEAAAAAAAVAATAPGPEMVERRGPGRPRTDGENVFTGQSKIYSYMSPNKCSGMRFPLQEENSVTHHEVKCQGKPLAGIYRKREEKRNAGNAVRSAMKSEEQKIKDARKGPLVPFPNQKSEAAEPPKTPPSSCDSTNAAIAKQALKKPIKGKQAPRKKAQGKTQQNRKLTDFYPVRRSSRKSKAELQSEERKRIDELIESGKEEGMKIDLIDGKGRGVIATKQFSRGDFVVEYHGDLIEITDAKKREALYAQDPSTGCYMYYFQYLSKTYCVDATRETNRLGRLINHSKCGNCQTKLHDIDGVRHLILIASRDIAAGEELLYDYGDRSKASIEAHPWLKH Predict reactive species |
| Full Name | SET domain containing (lysine methyltransferase) 8 |
| Calculated Molecular Weight | 43 kDa |
| Observed Molecular Weight | 43 kDa |
| GenBank Accession Number | BC050346 |
| Gene Symbol | SETD8 |
| Gene ID (NCBI) | 387893 |
| RRID | AB_2878006 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9NQR1 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
SETD8, also known as Set8, PR-Set7, or KMT5A, is a member of the SET domain family of histone lysine methyltransferases and, to date, the only known enzyme that catalyzes monomethylation of H4K20 in eukaryotes, which is involved in DNA damage response, mitotic condensation, and DNA replication. SETD8 is overexpressed in various types of tumors, such as bladder cancer, non-small cell lung carcinoma, and small cell lung carcinoma, and is associated with a shorter survival time for gastric, esophageal, and prostate cancer patients.
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for SETD8 antibody 14063-1-AP | Download protocol |
| IHC protocol for SETD8 antibody 14063-1-AP | Download protocol |
| IP protocol for SETD8 antibody 14063-1-AP | Download protocol |
| WB protocol for SETD8 antibody 14063-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
Anti-SETD8 (catalog no. 14063-1-AP) has 31 total citations. Journals include Cell Death & Differentiation, Cell Death & Disease, Pharmacological Research, Cancer Letters, International Journal of Biological Sciences, Journal of Translational Medicine, Journal of Medicinal Chemistry, Molecular Therapy Oncology, and others. Research fields span diabetic nephropathy, high glucose-induced endothelial inflammation, cancer (pancreatic, colorectal, gastric, cervical, breast, glioblastoma), ferroptosis, epigenetic regulation, cardiovascular disease, EMT, and chemoresistance.
| Species | Application | Title |
|---|---|---|
Exp Mol Med SET8 suppression mediates high glucose-induced vascular endothelial inflammation via the upregulation of PTEN.
| ||
Cell Death Differ SETD8-mediated mono-methylation of YAP at K76 promotes K48-linked polyubiquitination and degradation to suppress colorectal cancer | ||
Cell Death Differ NAT10 regulates heart development and function by maintaining the expression of genes related to fatty acid β-oxidation and heart contraction | ||
Cell Death Dis High glucose mediates NLRP3 inflammasome activation via upregulation of ELF3 expression. | ||
Cell Death Dis The CREB/KMT5A complex regulates PTP1B to modulate high glucose-induced endothelial inflammatory factor levels in diabetic nephropathy.
| ||
Pharmacol Res Discovery of KMT5A repressed miR-99b cluster with potential to restore chemotherapy sensitivity in gastric cancer by regulating mitochondrial complex II and affecting OXPHOS. |
