Tested Applications
| Positive WB detected in | human skeletal muscle tissue, HepG2 cells |
| Positive IP detected in | HepG2 cells |
| Positive IHC detected in | human gliomas tissue, human hepatocirrhosis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
14132-1-AP targets GFPT1 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, zebrafish |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag5312 Product name: Recombinant human GFPT1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-350 aa of BC045641 Sequence: MCGIFAYLNYHVPRTRREILETLIKGLQRLEYRGYDSAGVGFDGGNDKDWEANACKIQLIKKKGKVKALDEEVHKQQDMDLDIEFDVHLGIAHTRWATHGEPSPVNSHPQRSDKNNEFIVIHNGIITNYKDLKKFLESKGYDFESETDTETIAKLVKYMYDNRESQDTSFTTLVERVIQQLEGAFALVFKSVHFPGQAVGTRRGSPLLIGVRSEHKLSTDHIPILYRTGKDKKGSCNLSRVDSTTCLFPVEEKAVEYYFASDASAVIEHTNRVIFLEDDDVAAVVDGRLSIHRIKRTAGDHPGRAVQTLQMELQQIMKGNFSSFMQKEIFEQPESVVNTMRGRVNFDDYT Predict reactive species |
| Full Name | glutamine-fructose-6-phosphate transaminase 1 |
| Calculated Molecular Weight | 681 aa, 77 kDa |
| Observed Molecular Weight | 77 kDa |
| GenBank Accession Number | BC045641 |
| Gene Symbol | GFPT1 |
| Gene ID (NCBI) | 2673 |
| RRID | AB_2110155 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q06210 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
GFPT1 (glutamine-fructose-6-phosphate transaminase 1) catalyzes the transfer of an amino group from glutamine onto fructose-6-phosphate, yielding glucosamine-6-phosphate (GlcN-6-P) and glutamate. GFPT1 is the key enzyme of the hexosamine pathway and is required for critical events in neuromuscular transmission. Mutations of GFPT1 cause congenital myasthenic syndromes (CMS). (21310273, 25765662)
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for GFPT1 antibody 14132-1-AP | Download protocol |
| IHC protocol for GFPT1 antibody 14132-1-AP | Download protocol |
| IP protocol for GFPT1 antibody 14132-1-AP | Download protocol |
| WB protocol for GFPT1 antibody 14132-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The GFPT1 Polyclonal antibody (Cat# 14132-1-AP) has 44 citations spanning journals including Cell Metabolism, Cell Research, Cancer Research, Nature Communications, Circulation Research, Cell Reports, Journal of Clinical Investigation, Human Molecular Genetics, and International Journal of Molecular Sciences. Research fields cover O-GlcNAcylation, hexosamine biosynthetic pathway, heart failure, hepatocellular carcinoma, renal cell carcinoma, diabetes, ferroptosis, preeclampsia, neuromuscular disorders, glycosylation, and mitochondrial metabolism.
| Species | Application | Title |
|---|---|---|
Cell Metab Protein O-GlcNAcylation and hexokinase mitochondrial dissociation drive heart failure with preserved ejection fraction | ||
Cell Metab High dietary fructose promotes hepatocellular carcinoma progression by enhancing O-GlcNAcylation via microbiota-derived acetate | ||
Cell Res Mannose antagonizes GSDME-mediated pyroptosis through AMPK activated by metabolite GlcNAc-6P | ||
Cancer Res MTHFD2 Enhances cMYC O-GlcNAcylation to Promote Sunitinib Resistance in Renal Cell Carcinoma | ||
Nat Commun Compensatory activity of the PC-ME1 metabolic axis underlies differential sensitivity to mitochondrial complex I inhibition
| ||
Nat Commun Tick extracellular vesicles enable arthropod feeding and promote distinct outcomes of bacterial infection. |
Reviews
What customers say
The single review for this product is a 5-star rating from an immunofluorescence application. The user stained mouse testis tissue at a 1:200 dilution, visualizing Gfpt1 in green alongside DAPI nuclear counterstain in blue. The result was well-received, indicating the antibody performed effectively for IF on mouse testis at this dilution.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Kristian (Verified Customer) (06-07-2021) | Mouse testis were stained for Gfpt1 (green) and DAPI (blue)
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