Tested Applications
| Positive WB detected in | HL-60 cells |
| Positive IHC detected in | human pancreas cancer tissue, human intrahepatic cholangiocarcinoma tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
14262-1-AP targets VPS36 in WB, IHC, IF, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag5549 Product name: Recombinant human VPS36 protein Source: e coli.-derived, T-HIS Tag: 6*His Domain: 1-386 aa of BC050439 Sequence: MDRFVWTSGLLEINETLVIQQRGVRIYDGEEKIKFDAGTLLLSTHRLIWRDQKNHECCMAILLSQIVFIEEQAAGIGKSAKIVVHLHPAPPNKEPGPFQSSKNSYIKLSFKEHGQIEFYRRLSEEMTQRRWENMPVSQSLQTNRGPQPGRIRAVGIVGIERKLEEKRKETDKNISEAFEDLSKLMIKAKEMVELSKSIANKIKDKQGDITEDETIRFKSYLLSMGIANPVTRETYGSGTQYHMQLAKQLAGILQVPLEERGGIMSLTEVYCLVNRARGMELLSPEDLVNACKMLEALKLPLRLRVFDSGVMVIELQSHKEEEMVASALETVSEKGSLTSEEFAKLVGMSVLLAKERLLLAEKMGHLCRDDSVEGLRFYPNLFMTQS Predict reactive species |
| Full Name | vacuolar protein sorting 36 homolog (S. cerevisiae) |
| Calculated Molecular Weight | 44 kDa |
| Observed Molecular Weight | 43 kDa, 65 kDa |
| GenBank Accession Number | BC050439 |
| Gene Symbol | VPS36 |
| Gene ID (NCBI) | 51028 |
| RRID | AB_2878036 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q86VN1 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for VPS36 antibody 14262-1-AP | Download protocol |
| WB protocol for VPS36 antibody 14262-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The VPS36 Polyclonal antibody (Cat# 14262-1-AP) has 4 total citations published in Developmental Cell, Journal of Cell Biology, Cells, and Journal of Neuroscience Research. The associated research fields include autophagosome formation and closure, microautophagy of ubiquitylated tau aggregates, autophagic dysfunction in Fabry disease–associated hypertrophic cardiomyopathy, and photoreceptor differentiation during late-stage neural development. Applications cited are WB and IF in human samples.
| Species | Application | Title |
|---|---|---|
Dev Cell Transferrin receptor controls both autophagosome formation and closure via phosphatidylinositol 3-phosphate synthesis | ||
Cells Generation of GLA-Knockout Human Embryonic Stem Cell Lines to Model Autophagic Dysfunction and Exosome Secretion in Fabry Disease-Associated Hypertrophic Cardiomyopathy. | ||
J Neurosci Res Top2b is involved in the formation of outer segment and synapse during late-stage photoreceptor differentiation by controlling key genes of photoreceptor transcriptional regulatory network. |
