Tested Applications
| Positive WB detected in | HEK-293 cells, HeLa cells, K-562 cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | mouse brain tissue, mouse heart tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | U-251 cells, HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
14388-1-AP targets Casein Kinase 1 Delta in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag5856 Product name: Recombinant human CSNK1D protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 103-373 aa of BC003558 Sequence: KTVLLLADQMISRIEYIHSKNFIHRDVKPDNFLMGLGKKGNLVYIIDFGLAKKYRDARTHQHIPYRENKNLTGTARYASINTHLGIEQSRRDDLESLGYVLMYFNLGSLPWQGLKAATKRQKYERISEKKMSTPIEVLCKGYPSEFATYLNFCRSLRFDDKPDYSYLRQLFRNLFHRQGFSYDYVFDWNMLKFGASRAADDAERERRDREERLRHSRNPATRGLPSTASGRLRGTQEVAPPTPLTPTSHTANTSPRPVSGMERERKVSMRL Predict reactive species |
| Full Name | casein kinase 1, delta |
| Calculated Molecular Weight | 47 kDa |
| Observed Molecular Weight | 47 kDa |
| GenBank Accession Number | BC003558 |
| Gene Symbol | CK1 Delta |
| Gene ID (NCBI) | 1453 |
| RRID | AB_2084484 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P48730 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
CSNK1D(casein kinase I isoform delta) is also named as HCKID, CKId and belongs to the casein kinase I subfamily. CKI is a ubiquitous enzyme which has been found in the cytosol, associated with membranes, and in the nucleus. It can phosphorylate a large number of cytosolic, membrane-associated, and nuclear proteins(PMID:8454611). CSNK1D is found to be associated generally with granulovacuolar bodies and tau-containing neurofibrillary tangles in AD, DS, PSP, PDC, PPND, and controls, and Pick bodies and ballooned neurons in PiD(PMID:10924763). It has 2 isoforms produced by alternative splicing.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for Casein Kinase 1 Delta antibody 14388-1-AP | Download protocol |
| IHC protocol for Casein Kinase 1 Delta antibody 14388-1-AP | Download protocol |
| IP protocol for Casein Kinase 1 Delta antibody 14388-1-AP | Download protocol |
| WB protocol for Casein Kinase 1 Delta antibody 14388-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The Casein Kinase 1δ (CK1δ) antibody, catalog number 14388-1-AP, has 21 citations. These appear in journals including Molecular Cancer, Nature Communications, Developmental Cell, Oncogene, Cell Chemical Biology, Leukemia, Journal of Cell Biology, Neurobiology of Disease, Journal of Virology, and Molecular and Cellular Biology, among others. Research fields span oncology (melanoma, hepatocellular carcinoma, lymphoma), neurodegeneration (TDP-43, ALS, Alzheimer's, Lewy bodies), cell biology (autophagy, ER exit sites), and infectious disease (HIV). Applications are mainly WB, IHC, and IP.
| Species | Application | Title |
|---|---|---|
Mol Cancer SPOP-mediated ubiquitination and degradation of PDK1 suppresses AKT kinase activity and oncogenic functions. | ||
Nat Commun Pharmacological targeting of casein kinase 1δ suppresses oncogenic NRAS-driven melanoma | ||
Dev Cell Mitotic ER Exit Site Disassembly and Reassembly Are Regulated by the Phosphorylation Status of TANGO1. | ||
Oncogene β-Trcp and CK1δ-mediated degradation of LZTS2 activates PI3K/AKT signaling to drive tumorigenesis and metastasis in hepatocellular carcinoma.
| ||
Cell Chem Biol CDK2 heterobifunctional degraders co-degrade CDK2 and cyclin E resulting in efficacy in CCNE1-amplified and overexpressed cancers | ||
Leukemia A highly selective and orally bioavailable casein kinase 1 alpha degrader through p53 signaling pathway targets B-cell lymphoma cells |
Reviews
What customers say
The single review for this antibody reports a 5-star rating from a Western Blot application. The user tested it at a 1:2000 dilution on MCF7 cells to detect CK1δ following siRNA knockdown of CK1δ, and the antibody successfully detected the target. Overall, the feedback is positive, confirming the antibody's performance in WB for detecting CK1δ protein expression.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Christina (Verified Customer) (11-28-2023) | I used the antibody to detect CKD1δ in WB after siRNA of CK1δ (MCF7 cells).
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