Tested Applications
| Positive WB detected in | HepG2 cells, SGC-7901 cells, Jurkat cells |
| Positive IP detected in | SGC-7901 cells |
| Positive IHC detected in | human endometrial cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
15355-1-AP targets RPS27 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag7601 Product name: Recombinant human RPS27 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-84 aa of BC002658 Sequence: MPLAKDLLHPSPEEEKRKHKKKRLVQSPNSYFMDVKCPGCYKITTVFSHAQTVVLCVGCSTVLCQPTGGKARLTEGCSFRRKQH Predict reactive species |
| Full Name | ribosomal protein S27 |
| Calculated Molecular Weight | 9 kDa |
| Observed Molecular Weight | 9 kDa |
| GenBank Accession Number | BC002658 |
| Gene Symbol | RPS27 |
| Gene ID (NCBI) | 6232 |
| RRID | AB_2180509 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P42677 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for RPS27 antibody 15355-1-AP | Download protocol |
| IHC protocol for RPS27 antibody 15355-1-AP | Download protocol |
| IP protocol for RPS27 antibody 15355-1-AP | Download protocol |
| WB protocol for RPS27 antibody 15355-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The RPS27 Polyclonal antibody (Cat# 15355-1-AP) has 7 citations published in Nature Communications, Brain, Cell Reports, Cancer Metabolism, Journal of Molecular Biology, BMC Cancer, and FEBS Letters. Research fields span ribosome biogenesis, protein translation regulation, neural stem cell differentiation, lung cancer, prostate cancer metastasis, protein quality control, and Kaposi's sarcoma development.
| Species | Application | Title |
|---|---|---|
Nat Commun A post-transcriptional program coordinated by CSDE1 prevents intrinsic neural differentiation of human embryonic stem cells. | ||
Brain GGC repeat expansion in NOTCH2NLC induces dysfunction in ribosome biogenesis and translation | ||
J Mol Biol Defective Human SRP Induces Protein Quality Control and Triggers Stress Response | ||
BMC Cancer Exploration of RNA-binding proteins identified RPS27 as a potential regulator associated with Kaposi's sarcoma development |
