Tested Applications
| Positive WB detected in | HeLa cells, A549 cells, HL-60 cells, Jurkat cells, MCF-7 cells |
| Positive IP detected in | Jurkat cells |
| Positive IHC detected in | mouse brain tissue, mouse heart tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
15468-1-AP targets TRADD in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag7768 Product name: Recombinant human TRADD protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-312 aa of BC004491 Sequence: MAAGQNGHEEWVGSAYLFVESSLDKVVLSDAYAHPQQKVAVYRALQAALAESGGSPDVLQMLKIHRSDPQLIVQLRFCGRQPCGRFLRAYREGALRAALQRSLAAALAQHSVPLQLELRAGAERLDALLADEERCLSCILAQQPDRLRDEELAELEDALRNLKCGSGARGGDGEVASAPLQPPVPSLSEVKPPPPPPPAQTFLFQGQPVVNRPLSLKDQQTFARSVGLKWRKVGRSLQRGCRALRDPALDSLAYEYEREGLYEQAFQLLRRFVQAEGRRATLQRLVEALEENELTSLAEDLLGLTDPNGGLA Predict reactive species |
| Full Name | TNFRSF1A-associated via death domain |
| Calculated Molecular Weight | 34 kDa |
| Observed Molecular Weight | 34 kDa |
| GenBank Accession Number | BC004491 |
| Gene Symbol | TRADD |
| Gene ID (NCBI) | 8717 |
| RRID | AB_2209035 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q15628 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for TRADD antibody 15468-1-AP | Download protocol |
| IP protocol for TRADD antibody 15468-1-AP | Download protocol |
| WB protocol for TRADD antibody 15468-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The Rabbit polyclonal antibody to TRADD (Cat# 15468-1-AP) has 15 citations across journals including Cell Research, Cell Reports, Free Radical Biology and Medicine, International Journal of Biological Sciences, Phytomedicine, Journal of Translational Medicine, Molecular Medicine, FEBS Journal, Journal of Ethnopharmacology, Microbiological Research, Journal of Cellular and Molecular Medicine, and Archives of Dermatological Research. Research fields span apoptosis, pyroptosis, inflammation, cancer, cardiovascular disease, neurology, and gastroenterology.
| Species | Application | Title |
|---|---|---|
Cell Res The metabolite α-KG induces GSDMC-dependent pyroptosis through death receptor 6-activated caspase-8. | ||
Int J Biol Sci Mannan-Binding Lectin Deficiency Limits Inflammation-induced Myeloid-Derived Suppressor Cells Expansion via Modulating Tumor Necrosis Factor Alpha-triggered Apoptosis. | ||
Phytomedicine Modified Jiaoqi Powder enhances epithelial autophagy against TNF-triggered apoptosis in chronic ulcerative colitis | ||
J Transl Med tRNA-derived fragment 3'tRF-AlaAGC modulates cell chemoresistance and M2 macrophage polarization via binding to TRADD in breast cancer | ||
Microbiol Res Caspase-8 drove apoptosis of BMECs to promote neutrophil infiltration and DE205B clearance in meningitis | ||
Mol Med Knockdown of circROBO2 attenuates acute myocardial infarction through regulating the miR-1184/TRADD axis. |
