Tested Applications
| Positive WB detected in | Jurkat cells, COLO 320 cells, Raji cells, U-87 MG cells |
| Positive IHC detected in | human lymphoma tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | Hela cells |
| Positive FC (Intra) detected in | HEK-293 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:10-1:100 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.50 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
15796-1-AP targets BAP29 in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag8505 Product name: Recombinant human BCAP29 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 118-241 aa of BC008478 Sequence: RRLVTLITQLAKELSNKGVLKTQAENTNKAAKKFMEENEKLKRILKSHGKDEECVLEAENKKLVEDQEKLKTELRKTSDALSKAQNDVMEMKMQSERLSKEYDQLLKEHSELQDRLERGNKKRL Predict reactive species |
| Full Name | B-cell receptor-associated protein 29 |
| Calculated Molecular Weight | 241 aa, 28 kDa |
| Observed Molecular Weight | 28 kDa |
| GenBank Accession Number | BC008478 |
| Gene Symbol | BAP29 |
| Gene ID (NCBI) | 55973 |
| RRID | AB_2065118 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9UHQ4 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for BAP29 antibody 15796-1-AP | Download protocol |
| IHC protocol for BAP29 antibody 15796-1-AP | Download protocol |
| WB protocol for BAP29 antibody 15796-1-AP | Download protocol |
| FC protocol for BAP29 antibody 15796-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The BAP29 Polyclonal antibody (Cat# 15796-1-AP) has 1 citation. It appears in Cell Reports (2024), in a study investigating how Legionella exploits host Rab GTPases and BAP31 to establish a unique endoplasmic reticulum niche. The research falls within the fields of host–pathogen interactions, ER biology, and infectious disease. The antibody was applied in Western blot (WB) for human samples.
| Species | Application | Title |
|---|---|---|
Reviews
What customers say
The single review for this product is positive, with a 5-star rating. The user performed a Western Blot on A549 cells at a 1:1000 dilution and reported that the protein was detected, though it ran slightly lower than the predicted 28 kDa size, appearing around 24 kDa. Overall, the antibody performed as expected with a minor discrepancy in molecular weight.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Xin (Verified Customer) (03-19-2021) | The protein ran slightly lower than the predicted size (28kDa), it was detected somewhere around 24kDa.
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