Tested Applications
| Positive WB detected in | mouse pancreas tissue, mouse stomach tissue, rat pancreas tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:12000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
15892-1-AP targets RNASE1 in WB, ELISA applications and shows reactivity with Mouse, rat, human samples.
| Tested Reactivity | Mouse, rat, human |
| Cited Reactivity | mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag8679 Product name: Recombinant human RNASE1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 25-156 aa of BC005324 Sequence: PSLGKESRAKKFQRQHMDSDSSPSSSSTYCNQMMRRRNMTQGRCKPVNTFVHEPLVDVQNVCFQEKVTCKNGQGNCYKSNSSMHITDCRLTNGSRYPNCAYRTSPKERHIIVACEGSPYVPVHFDASVEDST Predict reactive species |
| Full Name | ribonuclease, RNase A family, 1 (pancreatic) |
| Calculated Molecular Weight | 156 aa, 18 kDa |
| Observed Molecular Weight | 18 kDa |
| GenBank Accession Number | BC005324 |
| Gene Symbol | RNASE1 |
| Gene ID (NCBI) | 6035 |
| RRID | AB_3085473 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P07998 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for RNASE1 antibody 15892-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The RNASE1 Polyclonal antibody (Cat# 15892-1-AP) has 1 total citation. It appears in American Journal of Physiology-Renal Physiology (IF 3.6), published in 2024. The cited study investigated NETs (neutrophil extracellular traps) formation and the renoprotective effect of degraded NETs in lupus nephritis, placing the research field in autoimmune nephrology and innate immune cell biology. The antibody was applied in Western Blot using mouse samples.
| Species | Application | Title |
|---|---|---|
Am J Physiol Renal Physiol Identification of NETs formation and the renoprotective effect of degraded NETs in lupus nephritis |
