Tested Applications
| Positive WB detected in | Raji cells, human liver tissue |
| Positive IHC detected in | human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
15976-1-AP targets PSMB10 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag8764 Product name: Recombinant human PSMB10 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-273 aa of BC017198 Sequence: MLKPALEPRGGFSFENCQRNASLERVLPGLKVPHARKTGTTIAGLVFQDGVILGADTRATNDSVVADKSCEKIHFIAPKIYCCGAGVAADAEMTTRMVASKMELHALSTGREPRVATVTRILRQTLFRYQGHVGASLIVGGVDLTGPQLYGVHPHGSYSRLPFTALGSGQDAALAVLEDRFQPNMTLEAAQGLLVEAVTAGILGDLGSGGNVDACVITKTGAKLLRTLSSPTEPVKRSGRYHFVPGTTAVLTQTVKPLTLELVEETVQAMEVE Predict reactive species |
| Full Name | proteasome (prosome, macropain) subunit, beta type, 10 |
| Calculated Molecular Weight | 273 aa, 29 kDa |
| Observed Molecular Weight | 29 kDa |
| GenBank Accession Number | BC017198 |
| Gene Symbol | PSMB10 |
| Gene ID (NCBI) | 5699 |
| RRID | AB_2171872 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P40306 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
The proteasome subunit, beta type 10 (PSMB10) gene regulated by interferon-gamma is a core part of the 26S proteasome complex, which is an important protein degrading system. Study identifies immunosubunit PSMB10 as a novel regulator that contributes to Ang II-induced atrial fibrillation (AF) and suggests that inhibition of PSMB10 may represent a potential therapeutic target for treating hypertensive AF. (PMID: 29507100, PMID: 16965406)
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for PSMB10 antibody 15976-1-AP | Download protocol |
| IHC protocol for PSMB10 antibody 15976-1-AP | Download protocol |
| WB protocol for PSMB10 antibody 15976-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The PSMB10 Polyclonal antibody (Cat# 15976-1-AP) has 7 total citations. These appear in journals including Journal of Translational Medicine, Cell Reports, Biochemical Pharmacology, International Journal of Molecular Sciences, Frontiers in Pharmacology, Scientific Reports, and Metabolic Brain Disease. Research fields span immunotherapy and bladder cancer, neuroimmunology, atrial fibrillation, pancreatic cancer antigen presentation, cardiac hypertrophy, ulcerative colitis, and Alzheimer's-like pathology in diabetic rats.
| Species | Application | Title |
|---|---|---|
J Transl Med Immunoproteasome subunits are novel signatures for predicting efficacy of immunotherapy in muscle invasive bladder cancer | ||
Cell Rep ECM29/proteasome-mediated self-antigen generation by CNS-resident neuroglia promotes regulatory T cell activation | ||
Biochem Pharmacol Melatonin inhibits angiotensin II-induced atrial fibrillation through preventing degradation of Ang II Type I Receptor-Associated Protein (ATRAP) | ||
Int J Mol Sci Gemcitabine Modulates HLA-I Regulation to Improve Tumor Antigen Presentation by Pancreatic Cancer Cells | ||
Front Pharmacol Selective Inhibition of the Immunoproteasome β5i Prevents PTEN Degradation and Attenuates Cardiac Hypertrophy. | ||
