Tested Applications
| Positive WB detected in | A431 cells, Jurkat cells |
| Positive IP detected in | Jurkat cells |
| Positive IHC detected in | mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
16035-1-AP targets SerpinB2/PAI-2 in WB, IHC, IP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag8906 Product name: Recombinant human SERPINB2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-415 aa of BC012609 Sequence: MEDLCVANTLFALNLFKHLAKASPTQNLFLSPWSISSTMAMVYMGSRGSTEDQMAKVLQFNEVGANAVTPMTPENFTSCGFMQQIQKGSYPDAILQAQAADKIHSSFRSLSSAINASTGNYLLESVNKLFGEKSASFREEYIRLCQKYYSSEPQAVDFLECAEEARKKIYSWVKTQTKGKIPNLLPEGSVDGDTRMVLVNAVYFKGKWKTPFEKKLNGLYPFRVNSAQRTPVQMMYLREKLNIGYIEDLKAQILELPYAGDVSMFLLLPDEIADVSTGLELLESEITYDKLNKWTSKDKMAEDEVEVYIPQFKLEEHYELRSILRSMGMEDAFNKGRANFSGMSERNDLFLSEVFHQAMVDVNEEGTEAAAGTGGVMTGRTGHGGPQFVADHPFLFLIMHKITNCILFFGRFSSP Predict reactive species |
| Full Name | serpin peptidase inhibitor, clade B (ovalbumin), member 2 |
| Calculated Molecular Weight | 415 aa, 47 kDa |
| Observed Molecular Weight | 45-47 kDa |
| GenBank Accession Number | BC012609 |
| Gene Symbol | SERPINB2 |
| Gene ID (NCBI) | 5055 |
| RRID | AB_2186180 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P05120 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
SerpinB2, a plasminogen activator inhibitor-2 (PAI-2), is a serine protease inhibitor of the serpin superfamily that inactivates tissue plasminogen activator (tPA) and urokinase plasminogen activator (uPA) (PMID: 7506655). SerpinB2 may have both pro-tumor and anti-tumor effects. High expression of tumor cell-associated SerpinB2 facilitates breast cancer cell survival and metastasis (PMID: 24581498; PMID: 28427146), while SerpinB2 expression by breast cancer cells significantly inhibits metastasis by inhibiting extracellular uPA (PMID: 24644264; PMID: 28346421).
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for SerpinB2/PAI-2 antibody 16035-1-AP | Download protocol |
| IP protocol for SerpinB2/PAI-2 antibody 16035-1-AP | Download protocol |
| WB protocol for SerpinB2/PAI-2 antibody 16035-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
SerpinB2/PAI-2 Polyclonal antibody (Cat# 16035-1-AP) has 12 citations published in journals including Molecular Cancer, Cancer Research, PNAS, Acta Biomaterialia, Oncotarget, Cell Oncology, Carcinogenesis, Frontiers in Endocrinology, Journal of Proteomics, PLoS ONE, and Aging. Research fields span cancer biology (hepatocellular, bladder, lung, and nasopharyngeal carcinomas), wound healing and angiogenesis, proteomics, diabetes and fatty liver disease, immunology, respiratory disease, and skin aging.
| Species | Application | Title |
|---|---|---|
Mol Cancer circPSD3 is a promising inhibitor of uPA system to inhibit vascular invasion and metastasis in hepatocellular carcinoma | ||
Cancer Res A Genome-Wide Synthetic Lethal Screen Identifies Spermidine Synthase as a Target to Enhance Erdafitinib Efficacy in FGFR-Mutant Bladder Cancer | ||
Acta Biomater A Hydrogel Tissue Adhesive Incorporating basic fibroblast growth factor-Loaded Liposomes Accelerates Cutaneous Wound Healing by Enhancing Cell Proliferation, Collagen Synthesis and Angiogenesis | ||
Proc Natl Acad Sci U S A CRISPR screens and quantitative proteomics reveal remodeling of the aryl hydrocarbon receptor-driven proteome through PARP7 activity | ||
Oncotarget Exposure to airborne PM2.5 suppresses microRNA expression and deregulates target oncogenes that cause neoplastic transformation in NIH3T3 cells. | ||
Front Endocrinol (Lausanne) Identification of biomarkers for the diagnosis of type 2 diabetes mellitus with metabolic associated fatty liver disease by bioinformatics analysis and experimental validation |
