Tested Applications
| Positive WB detected in | human saliva |
| Positive IHC detected in | human tonsillitis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
16078-1-AP targets TCN1 in WB, IHC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag9072 Product name: Recombinant human TCN1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-339 aa of BC018632 Sequence: SRLSDVSSGELALIILALGVCRNAEENLIYDYHLIDKLENKFQAEIENMEAHNGTPLTNYYQLSLDVLALCLFNGNYSTAEVVNHFTPENKNYYFGSQFSVDTGAMAVLALTCVKKSLINGQIKADEGSLKNISIYTKSLVEKILSEKKENGLIGNTFSTGEAMQALFVSSDYYNENDWNCQQTLNTVLTEISQGAFSNPNAAAQVLPALMGKTFLDINKDSSCVSASGNFNISADEPITVTPPDSQSYISVNYSVRINETYFTNVTVLNGSVFLSVMEKAQKMNDTIFGFTMEERSWGPYITCIQGLCANNNDRTYWELLSGGEPLSQGAGSYVVRNG Predict reactive species |
| Full Name | transcobalamin I (vitamin B12 binding protein, R binder family) |
| Calculated Molecular Weight | 433 aa, 48 kDa |
| Observed Molecular Weight | 65-70 kDa |
| GenBank Accession Number | BC018632 |
| Gene Symbol | TCN1 |
| Gene ID (NCBI) | 6947 |
| RRID | AB_2878215 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P20061 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for TCN1 antibody 16078-1-AP | Download protocol |
| WB protocol for TCN1 antibody 16078-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The TCN1 Polyclonal antibody (Cat# 16078-1-AP) has 6 citations published in Advanced Science, Science Advances, International Immunopharmacology, Journal of Inflammation Research, Pathology Research and Practice, and Journal of Molecular Histology. Research fields span pancreatic ductal adenocarcinoma (perineural invasion), psoriasis (autoimmunity and NF-κB signaling), sepsis-induced acute lung injury, and non-small cell lung cancer (glycolysis and histone lactylation). Applications include WB and IHC in human samples.
| Species | Application | Title |
|---|---|---|
Adv Sci (Weinh) Vitamin D Binding Protein, a Ligand of Integrin beta 1, Motivates Both Tumor Cells and Schwann Cells to Promote Perineural Invasion in Pancreatic Ductal Adenocarcinoma | ||
Sci Adv Transcobalamin 1 is a keratinocyte-derived autoantigen driving psoriasis through T helper 17 cell activation. | ||
Int Immunopharmacol TCN1 as an inflammatory regulator in psoriasis: Activation of the NF-κB pathway and potential therapeutic target | ||
J Inflamm Res Identification of Biomarkers for Sepsis-Induced Acute Lung Injury Through Bioinformatics and Machine Learning Approaches, with Experimental Validation. | ||
Pathol Res Pract TCN1 knockdown inhibits the progression and glycolysis of non-small cell lung cancer via regulating B3GNT3. | ||
J Mol Histol Histone H3K18 lactylation promotes malignancy progression in non-small cell lung cancer through TCN1 upregulation. |
