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GTPBP9 Polyclonal antibody

GTPBP9 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 16371-1-AP

Publications (6)

Reviews (2)

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Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

OLA1, DNA damage-regulated overexpressed in cancer 45, GTP binding protein 9, GTP-binding protein 9, Obg like ATPase 1

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

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Filter: AllWBIHCIF/ICC

WB analysis of Jurkat using 16371-1-AP
Jurkat cells were subjected to SDS PAGE followed by western blot with 16371-1-AP (GTPBP9 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of HepG2 using 16371-1-AP
WB result of GTPBP9 antibody (16371-1-AP; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GTPBP9 transfected HepG2 cells.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of HepG2 using 16371-1-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 16371-1-AP (GTPBP9 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human liver cancer using 16371-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16371-1-AP (GTPBP9 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human liver cancer using 16371-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16371-1-AP (GTPBP9 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human colon cancer using 16371-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer using 16371-1-AP (GTPBP9 antibody) at dilution of 1:50 (under 10x lens).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human colon cancer using 16371-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer using 16371-1-AP (GTPBP9 antibody) at dilution of 1:50 (under 40x lens).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IF Staining of MCF-7 using 16371-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed MCF-7 cells using 16371-1-AP (GTPBP9 antibody) at dilution of 1:200 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Overview

Tested Applications
Product Information
Publications (6)
Protocols
Reviews (2)

Print datasheet

MSDS SDS

Tested Applications

Positive WB detected in	Jurkat cells, HepG2 cells
Positive IHC detected in	human liver cancer tissue, human colon cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	MCF-7 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16371-1-AP targets GTPBP9 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag9283 Product name: Recombinant human OLA1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-250 aa of BC013925 Sequence: MPPKKGGDGIKPPPIIGRFGTSLKIGIVGLPNVGKSTFFNVLTNSQASAENFPFCTIDPNESRVPVPDERFDFLCQYHKPASKIPAFLNVVDIAGLVKGAHNGQGLGNAFLSHISACDGIFHLTRAFEDDDITHVEGSVDPIRDIEIIHEELQLKDEEMIGPIIDKLEKVAVRGGDKKLKPEYDIMCKVKSWVIDQKKPVRFYHDWNDKEIEVLNKHLFLTSKPMVYLVNLSEKDYIRKKNKWLLESTDN Predict reactive species
Full Name	Obg-like ATPase 1
Calculated Molecular Weight	396aa,45 kDa; 278aa,31 kDa
Observed Molecular Weight	45 kDa
GenBank Accession Number	BC013925
Gene Symbol	OLA1
Gene ID (NCBI)	29789
RRID	AB_2157087
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q9NTK5
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
IF protocol for GTPBP9 antibody 16371-1-AP	Download protocol
IHC protocol for GTPBP9 antibody 16371-1-AP	Download protocol
WB protocol for GTPBP9 antibody 16371-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

What published studies show

The GTPBP9 (OLA1) Polyclonal antibody (Cat# 16371-1-AP) has 6 citations published in iScience, J Cell Sci, PLoS One, Clin Exp Pharmacol Physiol, bioRxiv, and medRxiv. The cited research spans cardiovascular disease (dilated cardiomyopathy, hypertrophic response, failing heart), viral immunology (HIV-1/STING pathway), and gut microbiota–related arrhythmia, with applications primarily in Western blot and immunofluorescence in human and mouse models.

Species	Application	Title
mouse	WB	iScience Aged gut microbiota promotes arrhythmia susceptibility via oxidative stress Authors - Zhi-Ping Fu View Article
human	WB	J Cell Sci HIV-1 p17 matrix protein enhances type I interferon responses through the p17-OLA1-STING axis Authors - Lianfei Zhang KO Validated View Article
human	WB	PLoS One Identification and development of Tetra-ARMS PCR-based screening test for a genetic variant of OLA1 (Tyr254Cys) in the human failing heart Authors - Praveen K Dubey View Article
human	WB	Clin Exp Pharmacol Physiol OBG-like ATPase 1 inhibition attenuates angiotensin II-induced hypertrophic response in human ventricular myocytes via GSK-3beta/beta-catenin signalling. Authors - Gayathri Narasimhan KD Validated View Article
mouse,human	WB	medRxiv Identification and development of Tetra-ARMS PCR-based screening test for a genetic variant of OLA1 (Tyr254Cys) in the human failing heart Authors - Praveen K Dubey View Article
mouse	WB,IF	bioRxiv Obg-like ATPase 1 Genetic Deletion Leads to Dilated Cardiomyopathy in Mice and Structural Changes in Drosophila Heart Authors - Praveen K Dubey View Article

Reviews

5.0 out of 5

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2 verified customer reviews

What customers say

Both reviews rate the product 5 stars for Western Blot. One user tested it on AC16 cells at a 1:1000 dilution and described the result as "excellent." The other user tested it on H9C2 cells at a 1:3000 dilution and rated it "Good." Overall, customers report strong performance and reliable band detection in WB applications across different cell types and dilutions.

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

S (Verified Customer) (03-20-2023)	excellent Applications: Western Blot Primary Antibody Dilution: 1000 Cell Tissue Type: AC16 GTPBP9 Antibody Western Blot validation (1000 dilution) in AC16 (Cat no:16371-1-AP)
p (Verified Customer) (05-27-2021)	Good Applications: Western Blot Primary Antibody Dilution: 1:3000 Cell Tissue Type: H9C2 GTPBP9 Antibody Western Blot validation (1:3000 dilution) in H9C2 (Cat no:16371-1-AP)