RBM38 Polyclonal antibody

RBM38 Polyclonal Antibody for WB, ELISA

Cat No. 16435-1-AP

Publications(1)

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, ELISA

Synonyms

CLL associated antigen KW 5, CLL-associated antigen KW-5, HSRNASEB, RNA binding motif protein 38, RNA binding protein 38

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

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Quantity

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Filter: AllWBIHC

WB analysis using 16435-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 16435-1-AP (RBM38 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human stomach cancer using 16435-1-AP
Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 16435-1-AP (RBM38 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of mouse kidney using 16435-1-AP
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 16435-1-AP (RBM38 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of mouse stomach using 16435-1-AP
Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 16435-1-AP (RBM38 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of rat kidney using 16435-1-AP
Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 16435-1-AP (RBM38 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of rat stomach using 16435-1-AP
Immunohistochemical analysis of paraffin-embedded rat stomach tissue slide using 16435-1-AP (RBM38 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of mouse stomach using 16435-1-AP
Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 16435-1-AP (RBM38 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of rat stomach using 16435-1-AP
Immunohistochemical analysis of paraffin-embedded rat stomach tissue slide using 16435-1-AP (RBM38 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

Overview

Tested Applications
Product Information
Publications (1)
Protocols

Print datasheet

MSDS SDS

Tested Applications

Positive WB detected in

HL-60 cells, MCF-7 cells, SMMC-7721 cells, rat heart tissue

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16435-1-AP targets RBM38 in WB, ELISA applications and shows reactivity with human, rat samples.

Tested Reactivity	human, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag9748 Product name: Recombinant human RBM38 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-216 aa of BC018711 Sequence: MHGSQKDTTFTKIFVGGLPYHTTDASLRKYFEGFGDIEEAVVITDRQTGKSRGYGFVTMADRAAAERACKDPNPIIDGRKANVNLAYLGAKPRSLQTGFAIGVQQLHPTLIQRTYGLTPHYIYPPAIVQPSVVIPAAPVPSLSSPYIEYTPASPAYAQYPPATYDQYPYAASPATAASFVGYSYPAAVPQALSAAAPAGTTFVQYQAPQLQPDRMQ Predict reactive species
Full Name	RNA binding motif protein 38
Calculated Molecular Weight	216 aa, 23 kDa
Observed Molecular Weight	25-31 kDa
GenBank Accession Number	BC018711
Gene Symbol	RBM38
Gene ID (NCBI)	55544
RRID	AB_3669244
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q9H0Z9
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.