Tested Applications
| Positive WB detected in | mouse liver tissue, human heart tissue, human liver tissue, rat liver tissue |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
16499-1-AP targets MCEE in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag9665 Product name: Recombinant human MCEE protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-176 aa of BC020825 Sequence: MARVLKAAAANAVGLFSRLQAPIPTVRASSTSQPLDQVTGSVWNLGRLNHVAIAVPDLEKAAAFYKNILGAQVSEAVPLPEHGVSVVFVNLGNTKMELLHPLGLDSPIAGFLQKNKAGGMHHICIEVDNINAAVMDLKKKKIRSLSEEVKIGAHGKPVIFLHPKDCGGVLVELEQA Predict reactive species |
| Full Name | methylmalonyl CoA epimerase |
| Calculated Molecular Weight | 176 aa, 19 kDa |
| Observed Molecular Weight | 15-19 kDa |
| GenBank Accession Number | BC020825 |
| Gene Symbol | MCEE |
| Gene ID (NCBI) | 84693 |
| RRID | AB_2143812 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q96PE7 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for MCEE antibody 16499-1-AP | Download protocol |
| WB protocol for MCEE antibody 16499-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The MCEE polyclonal antibody (Cat# 16499-1-AP) has 2 citations. One appeared in Nature Metabolism (2022), studying altered propionate metabolism in tumour progression and aggressiveness. The other was published in BBA Molecular Basis of Disease (2023), investigating ACSS3's role in epithelial cell metabolic homeostasis and pulmonary fibrosis. Both citations used the antibody for Western blot in human samples, spanning research fields of cancer metabolism and lung disease.
| Species | Application | Title |
|---|---|---|
Nat Metab Altered propionate metabolism contributes to tumour progression and aggressiveness. | ||
Biochim Biophys Acta Mol Basis Dis ACSS3 regulates the metabolic homeostasis of epithelial cells and alleviates pulmonary fibrosis |
