Tested Applications
| Positive WB detected in | HEK-293 cells, HepG2 cells, L02 cells, MCF-7 cells |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
16563-1-AP targets HAO2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag9838 Product name: Recombinant human HAO2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-351 aa of BC020863 Sequence: MSLVCLTDFQAHAREQLSKSTRDFIEGGADDSITRDDNIAAFKRIRLRPRYLRDVSEVDTRTTIQGEEISAPICIAPTGFHCLVWPDGEMSTARAAQAAGICYITSTFASCSLEDIVIAAPEGLRWFQLYVHPDLQLNKQLIQRVESLGFKALVITLDTPVCGNRRHDIRNQLRRNLTLTDLQSPKKGNAIPYFQMTPISTSLCWNDLSWFQSITRLPIILKGILTKEDAELAVKHNVQGIIVSNHGGRQLDEVLASIDALTEVVAAVKGKIEVYLDGGVRTGNDVLKALALGAKCIFLGRPILWGLACKGEHGVKEVLNILTNEFHTSMALTGCRSVAEINRNLVQFSRL Predict reactive species |
| Full Name | hydroxyacid oxidase 2 (long chain) |
| Calculated Molecular Weight | 351 aa, 39 kDa |
| Observed Molecular Weight | 40 kDa |
| GenBank Accession Number | BC020863 |
| Gene Symbol | HAO2 |
| Gene ID (NCBI) | 51179 |
| RRID | AB_3669250 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9NYQ3 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for HAO2 antibody 16563-1-AP | Download protocol |
| WB protocol for HAO2 antibody 16563-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The HAO2 Polyclonal antibody (Cat# 16563-1-AP) has 1 citation. It was cited in Arthritis Research & Therapy (IF 5.4), a study investigating ferroptosis-associated molecular subtypes and immunological characteristics in lupus nephritis using artificial neural network learning. The research field encompasses ferroptosis, autoimmune disease (lupus nephritis), and immunology. The antibody was applied via IHC in human samples.
| Species | Application | Title |
|---|---|---|
Arthritis Res Ther Investigation of ferroptosis-associated molecular subtypes and immunological characteristics in lupus nephritis based on artificial neural network learning |
