Tested Applications
| Positive WB detected in | mouse testis tissue, human brain tissue |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
16590-1-AP targets MED31 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag9008 Product name: Recombinant human MED31 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-131 aa of BC012539 Sequence: MAAAVAMETDDAGNRLRFQLELEFVQCLANPNYLNFLAQRGYFKDKAFVNYLKYLLYWKDPEYAKYLKYPQCLHMLELLQYEHFRKELVNAQCAKFIDEQQILHWQHYSRKRMRLQQALAEQQQQNNTSGK Predict reactive species |
| Full Name | mediator complex subunit 31 |
| Calculated Molecular Weight | 131 aa, 16 kDa |
| Observed Molecular Weight | 16 kDa |
| GenBank Accession Number | BC012539 |
| Gene Symbol | MED31 |
| Gene ID (NCBI) | 51003 |
| RRID | AB_2142447 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9Y3C7 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
MED31, also named as SOH1 or CGI-125, is a 131 amino acid protein, which belongs to the Mediator complex subunit 31 family. MED31 localizes in the nucleus. MED31 as a component of the Mediator complex, a coactivator is involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. MED31 functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for MED31 antibody 16590-1-AP | Download protocol |
| WB protocol for MED31 antibody 16590-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The MED31 Polyclonal antibody (Cat# 16590-1-AP) has 1 total citation. It was published in Developmental Biology (2010), in a study investigating the role of the Mediator complex protein Med31 in embryonic growth and cell proliferation during mammalian development. The research field is developmental biology, specifically focusing on transcriptional regulation and embryogenesis. The cited application was Western Blot in mouse.
| Species | Application | Title |
|---|---|---|
Dev Biol The Mediator complex protein Med31 is required for embryonic growth and cell proliferation during mammalian development. |
Reviews
What customers say
Both reviewers gave this antibody a 5-star rating for Western Blot. One user tested it at 1:500 dilution on mouse cardiomyocytes and reported very clear bands. Another user tested it at 1:1000 dilution on mouse heart tissue and described the result as a good band. Both reviews were from the same lot (00007933) and were posted in 2021. Overall, users are satisfied with the specificity and signal quality of this antibody in WB applications.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Anh (Verified Customer) (07-15-2021) | very clear bands
|
siting (Verified Customer) (02-17-2021) | good band
 |
