Tested Applications
| Positive WB detected in | MCF-7 cells, HeLa cells |
| Positive IP detected in | HeLa cells |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
16633-1-AP targets RTF2 in WB, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag9972 Product name: Recombinant human C20orf43 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-306 aa of BC003359 Sequence: MGCDGGTIPKRHELVKGPKKVEKVDKDAELVAQWNYCTLSQEILRRPIVACELGRLYNKDAVIEFLLDKSAEKALGKAASHIKSIKNVTELKLSDNPAWEGDKGNTKGDKHDDLQRARFICPVVGLEMNGRHRFCFLRCCGCVFSERALKEIKAEVCHTCGAAFQEDDVIVLNGTKEDVDVLKTRMEERRLRAKLEKKTKKPKAAESVSKPDVSEEAPGPSKVKTGKPEEASLDSREKKTNLAPKSTAMNESSSGKAGKPPCGATKRSIADSEESEAYKSLFTTHSSAKRSKEESAHWVTHTSYCF Predict reactive species |
| Full Name | chromosome 20 open reading frame 43 |
| Calculated Molecular Weight | 34 kDa |
| Observed Molecular Weight | 35-40 kDa |
| GenBank Accession Number | BC003359 |
| Gene Symbol | RTF2 |
| Gene ID (NCBI) | 51507 |
| RRID | AB_2256547 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9BY42 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
The function of C20orf43 remains largely unknown. Catalog#16633-1-AP is a rabbit polyclonal antibody raised against the full-length of human C20orf43. The MW of this protein is 40 kDa, and this antibody specially recognises the 40 kDa protein.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for RTF2 antibody 16633-1-AP | Download protocol |
| IP protocol for RTF2 antibody 16633-1-AP | Download protocol |
| WB protocol for RTF2 antibody 16633-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The anti-RTF2 antibody (Cat# 16633-1-AP) has 2 citations. It appears in Molecular Cell and bioRxiv, both studying RTF2's role in replication timing factor function at stalled replisomes. The research fields encompass genome integrity maintenance, DNA replication repriming, and replisome biology in human and mouse systems.
| Species | Application | Title |
|---|---|---|
Mol Cell Removal of RTF2 from Stalled Replisomes Promotes Maintenance of Genome Integrity.
| ||
bioRxiv RTF2 controls replication repriming and ribonucleotide excision at the replisome
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