Tested Applications
| Positive WB detected in | MCF-7 cells |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
16916-1-AP targets KRCC1 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag10157 Product name: Recombinant human KRCC1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-187 aa of BC107580 Sequence: MKHSKKTYDSFQDELEDYIKVQKARGLEPKTCFRKMKGDYLETCGYKGEVNSRPTYRMFDQRLPSETIQTYPRSCNIPQTVENRLPQWLPAHDSRLRLDSLSYCQFTRDCFSEKPVPLNFNQQEYICGSHGVEHRVYKHFSSDNSTSTHQASHKQIHQKRKRHPEEGREKSEEERSKHKRKKKLRGN Predict reactive species |
| Full Name | lysine-rich coiled-coil 1 |
| Calculated Molecular Weight | 187 aa, 22 kDa |
| Observed Molecular Weight | 31-35 kDa |
| GenBank Accession Number | BC107580 |
| Gene Symbol | KRCC1 |
| Gene ID (NCBI) | 51315 |
| RRID | AB_2878331 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9NPI7 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for KRCC1 antibody 16916-1-AP | Download protocol |
| WB protocol for KRCC1 antibody 16916-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The KRCC1 Polyclonal antibody (Cat# 16916-1-AP) has 2 citations. One appeared in Nucleic Acids Research (2022), investigating KRCC1 as a modulator of the DNA damage response. The other was published in FASEB Journal (2019), exploring KRCC1 as a potential therapeutic target in ovarian cancer. Both studies used the antibody for Western blotting on human samples, with one also employing immunofluorescence. The associated research fields are DNA damage response and ovarian cancer biology.
| Species | Application | Title |
|---|---|---|
