Tested Applications
| Positive WB detected in | PC-3 cells, HepG2 cells, L02 cells, NIH/3T3 cells, PC-12 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
17029-1-AP targets RPL18 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, pig |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag10474 Product name: Recombinant human RPL18 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-186 aa of BC009708 Sequence: MGVDIRHNKDRKVRRKEPKSQDIYLRLLVKLYRFLARRTNSTFNQVVLKRLFMSRTNRPPLSLSRMIRKMKLPGRENKTAVVVGTITDDVRVQEVPKLKVCALRVTSRARSRILRAGGKILTFDQLALDSPKGCGTVLLSGPRKGREVYRHFGKAPGTPHSHTKPYVRSKGRKFERARGRRASRGY Predict reactive species |
| Full Name | ribosomal protein L18 |
| Calculated Molecular Weight | 188 aa, 22 kDa |
| Observed Molecular Weight | 22-26 kDa |
| GenBank Accession Number | BC009708 |
| Gene Symbol | RPL18 |
| Gene ID (NCBI) | 6141 |
| RRID | AB_2878340 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q07020 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for RPL18 antibody 17029-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The RPL18 Polyclonal antibody (Cat# 17029-1-AP) has 5 total citations. These appear in Signal Transduction and Targeted Therapy, Biomaterials, iScience, Virus Research, and bioRxiv. Associated research fields include hepatocellular carcinoma and lenvatinib resistance, personalized tumor vaccines, leukemia cell survival in bone marrow, PEDV viral replication, and CML imatinib resistance.
| Species | Application | Title |
|---|---|---|
Signal Transduct Target Ther Activation of Nerve Growth Factor signaling limits the response to lenvatinib in hepatocellular carcinoma | ||
Biomaterials P-Pev: micelle-like complexes transformed from tumor extracellular vesicles by PEG-PE for personalized therapeutic tumor vaccine | ||
iScience TIAR and FMRP shape pro-survival nascent proteome of leukemia cells in the bone marrow microenvironment | ||
Virus Res Up-regulated 60S ribosomal protein L18 in PEDV N protein-induced S-phase arrested host cells promotes viral replication
| ||
bioRxiv TIAR-dependent coordination of alternative splicing and lipid peroxidation is required for CML cell resistance to imatinib in the bone marrow stroma. |
Reviews
What customers say
The single review for this product reports a positive Western Blot experience. The user found that the antibody produced an appropriately sized band when used at a 1:1000 dilution on mouse T cells. The product received a 4 out of 5 rating, indicating overall satisfaction with its performance in detecting the target protein under these conditions.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Katie (Verified Customer) (05-23-2022) | Gives appropriate sized band in western blot.
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