Tested Applications
| Positive WB detected in | rat liver tissue, mouse kidney tissue, mouse liver tissue |
| Positive IHC detected in | human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:6000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
17356-1-AP targets CHDH in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag10614 Product name: Recombinant human CHDH protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 244-594 aa of BC034502 Sequence: YLHPALSRTNLKAEAETLVSRVLFEGTRAVGVEYVKNGQSHRAYASKEVILSGGAINSPQLLMLSGIGNADDLKKLGIPVVCHLPGVGQNLQDHLEIYIQQACTRPITLHSAQKPLRKVCIGLEWLWKFTGEGATAHLETGGFIRSQPGVPHPDIQFHFLPSQVIDHGRVPTQQEAYQVHVGPMRGTSVGWLKLRSANPQDHPVIQPNYLSTETDIEDFRLCVKLTREIFAQEALAPFRGKELQPGSHIQSDKEIDAFVRAKADSAYHPSCTCKMGQPSDPTAVVDPQTRVLGVENLRVVDASIMPSMVSGNLNAPTIMIAEKAADIIKGQPALWDKDVPVYKPRTLATQR Predict reactive species |
| Full Name | choline dehydrogenase |
| Calculated Molecular Weight | 594 aa, 65 kDa |
| Observed Molecular Weight | 60 kDa |
| GenBank Accession Number | BC034502 |
| Gene Symbol | CHDH |
| Gene ID (NCBI) | 55349 |
| RRID | AB_2276253 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q8NE62 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
CHDH(Choline dehydrogenase, mitochondrial) is also named as CDH,CHD and belongs to the GMC oxidoreductase family. CHDH is a member of the glucose-methanol-choline family of oxidoreductases, with each member containing a consensus FAD-binding domain. Many members of this oxidoreductase family are soluble proteins but mammalian CHDH is associated with the inner mitochondrial membrane(Handbook of vitamins,Robert B,2007). The full length protein(65 kDa) has a transit peptide of 29 amino acid and the mature CHDH is appropriately located in the inner mitochondria membrane(PMID:12951056). 17356-1-AP can also recognize the 45 kDa isoform X3 of CHDH.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for CHDH antibody 17356-1-AP | Download protocol |
| WB protocol for CHDH antibody 17356-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
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| Species | Application | Title |
|---|---|---|
Sci Adv Maintaining hypoxia environment of subchondral bone alleviates osteoarthritis progression | ||
Transl Oncol Quantitative proteomic profiling of hepatocellular carcinoma at different serum alpha-fetoprotein level. | ||
Poult Sci Global and mitochondrial choline dehydrogenase contribute to goose fatty liver formation by differentially regulating energy metabolism, autophagy and oxidative stress related pathways. | ||
J Biol Chem Betaine is accumulated via transient choline dehydrogenase activation during Mouse oocyte meiotic maturation.
|
Reviews
What customers say
Both reviews report successful Western Blot performance. One user detected a clear band at approximately 60 kDa in a T-ALL cell line using 30 µg of protein at a 1:2000 dilution, rating the product 4 out of 5 stars. The other user achieved strong results on mouse liver lysate (40 µg) at a 1:1000 dilution with an overnight incubation at 4 °C, giving a 5-star rating. Overall, customers found the antibody reliable for WB at dilutions between 1:1000 and 1:2000, with clear and specific band detection in both cell line and tissue samples.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Wojciech (Verified Customer) (03-23-2023) | A band was detected at around 60 kDa. 30 ug of protein was loaded and probed with the antibody at 1:2000 dilution.
|
Hua (Verified Customer) (12-04-2018) | 40 ug of mouse liver lysate was loaded onto the gel. The incubation time is over night at 4oC.
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