Tested Applications
| Positive WB detected in | A431 cells, A549 cells, HepG2 cells, Jurkat cells, MCF-7 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
18126-1-AP targets PARP15 in WB, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag12702 Product name: Recombinant human PARP15 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 96-444 aa of BC101701 Sequence: NRKSGVSRAILEGAGQAVESECAVLAAQPHRDFIITPGGCLKCKIIIHVPGGKDVRKTVTSVLEECEQRKYTSVSLPAIGTGNAGKNPITVADNIIDAIVDFSSQHSTPSLKTVKVVIFQPELLNIFYDSMKKRDLSASLNFQSTFSMTTCNLPEHWTDMNHQLFCMVQLEPGQSEYNTIKDKFTRTCSSYAIEKIERIQNAFLWQSYQVKKRQMDIKNDHKNNERLLFHGTDADSVPYVNQHGFNRSCAGKNAVSYGKGTYFAVDASYSAKDTYSKPDSNGRKHMYVVRVLTGVFTKGRAGLVTPPPKNPHNPTDLFDSVTNNTRSPKLFVVFFDNQAYPEYLITFTA Predict reactive species |
| Full Name | poly (ADP-ribose) polymerase family, member 15 |
| Calculated Molecular Weight | 444aa,50 kDa; 656aa,73 kDa |
| Observed Molecular Weight | 50 kDa |
| GenBank Accession Number | BC101701 |
| Gene Symbol | PARP15 |
| Gene ID (NCBI) | 165631 |
| RRID | AB_2158519 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q460N3 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for PARP15 antibody 18126-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The PARP15 Polyclonal antibody (Cat# 18126-1-AP) has 2 citations. These appear in Communications Biology (2026), studying viral macrodomain mono-ADP-ribosylhydrolase activity in cells, and Nucleic Acids Research (2026), investigating functional roles of viral RNA ribosylation in mammalian cells. Both citations relate to viral ADP-ribosylation biology and host-pathogen interactions.
| Species | Application | Title |
|---|---|---|
Commun Biol Screening assay to monitor mono-ADP-ribosylhydrolase activity of viral macrodomains in cells. | ||
Reviews
What customers say
The single review for this product is a 5-star rating from a Western Blot application using a 1:6000 dilution on HEK293 and U2OS cells. The reviewer described it as a "good working antibody for WB," indicating reliable performance under their experimental conditions. Overall, the feedback is positive, confirming the antibody's utility for detecting its target in cell lysates by Western blot.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Lisa (Verified Customer) (07-23-2025) | Good working antibody for WB.
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