Tested Applications
| Positive WB detected in | HEK-293 cells, SH-SY5Y cells, PC-3 cells, C6 cells, mouse kidney tissue, rat tissue, NIH/3T3 cells |
| Positive IP detected in | mouse brain tissue, HEK-293 cells |
| Positive IHC detected in | human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | MCF-7 cells |
| Positive FC (Intra) detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
18295-1-AP targets HRAS-Specific in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | v-Ha-ras Harvey rat sarcoma viral oncogene homolog |
| Calculated Molecular Weight | 21 kDa |
| Observed Molecular Weight | 21 kDa |
| GenBank Accession Number | BC006499 |
| Gene Symbol | HRAS |
| Gene ID (NCBI) | 3265 |
| RRID | AB_2121046 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P01112 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
HRAS, also named as c-bas/has and RASH1, is v-Ha-ras Harvey rat sarcoma viral oncogene homolog from the mammalian ras gene family and it is a member of the small GTPase superfamily. RAS proteins are involved in signal transduction pathways, and bind GDP/GTP and possess intrinsic GTPase activity. Defects in HRAS are a cause of cancer. The antibody is specific to HRAS, can't recognize NRAS and KRAS.
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for HRAS-Specific antibody 18295-1-AP | Download protocol |
| IF protocol for HRAS-Specific antibody 18295-1-AP | Download protocol |
| IHC protocol for HRAS-Specific antibody 18295-1-AP | Download protocol |
| IP protocol for HRAS-Specific antibody 18295-1-AP | Download protocol |
| WB protocol for HRAS-Specific antibody 18295-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The HRAS-Specific Polyclonal antibody (Cat# 18295-1-AP) has accumulated 90 citations. Publications span high-impact journals including Nature, Cancer Cell, Nature Cancer, Cancer Research, Nature Communications, Molecular Cell, PNAS, Oncogene, Cell Reports, Science Translational Medicine, and Cell Chemical Biology, among over 50 journals. Research fields primarily cover oncology (RAS-mutant cancers, targeted therapy, PROTAC degraders), RAS/MAPK signal transduction, drug discovery, neuroscience (Alzheimer's disease, stroke), and immunology.
| Species | Application | Title |
|---|---|---|
Nat Cancer Co-targeting SOS1 enhances the antitumor effects of KRASG12C inhibitors by addressing intrinsic and acquired resistance | ||
Nat Aging Aging-associated lncRNAs are evolutionarily conserved and participate in NFκB signaling | ||
Cancer Res Combined Inhibition of HRAS and MEK Induces Tumor Regression and Restores Myogenic Differentiation in HRAS-Mutant Rhabdomyosarcoma. | ||
Cancer Res Oncogenic Transformation Drives DNA Methylation Loss and Transcriptional Activation of Transposable Element Loci |
Reviews
What customers say
Both reviewers used this antibody for Western Blot at a 1:1000 dilution. One user reported excellent performance on CRC cell lines, noting it successfully replaced a failed Santa Cruz HRAS antibody and was used in a published paper. The other user confirmed clear detection of HRAS at the expected ~20 kDa on HEK cells. Overall, the antibody is well-received for reliable HRAS detection in Western Blot applications.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Stella (Verified Customer) (08-26-2026) | Since the HRAS antibody from Santa Cruz failed, we have ordered your product for our publication (doi: 10.1038/s41419-026-08434-5). This was very successful for us and we have also published our blots using your antibody.
|
Benjamin (Verified Customer) (01-10-2020) | Identified HRAS around 20kDa mark on western blot.
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