GPR142 Polyclonal antibody

GPR142 Polyclonal Antibody for WB, ELISA

Cat No. 18820-1-AP

Publications (1)

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Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

G protein coupled receptor 142, GPR142, PGR2

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

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WB analysis of SH-SY5Y using 18820-1-AP
SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 18820-1-AP (GPR142 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of SH-SY5Y using 18820-1-AP
SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 18820-1-AP (GPR142 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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What's the best dilution for GPR142 in ?

Overview

Tested Applications
Product Information
Publications (1)
Protocols

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MSDS SDS

Tested Applications

Positive WB detected in

SH-SY5Y cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

18820-1-AP targets GPR142 in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Peptide Predict reactive species
Full Name	G protein-coupled receptor 142
Calculated Molecular Weight	462 aa, 51 kDa
Observed Molecular Weight	51-55 kDa
GenBank Accession Number	BC152987
Gene Symbol	GPR142
Gene ID (NCBI)	350383
RRID	AB_10597384
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q7Z601
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GPR142, also named as PGR2, belongs to the G-protein coupled receptor 1 family. It is an orphan receptor. This antibody recognizes the 51-kDa GRP142.

Protocols

Product Specific Protocols
WB protocol for GPR142 antibody 18820-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

What published studies show

The GPR142 Polyclonal antibody (Cat# 18820-1-AP) has 1 total citation. It appears in BMC Genomics, in a study integrating proteome and transcriptome analyses to investigate fiber diameter traits in Tibetan cashmere goats. The research field encompasses genomics, proteomics, and animal science, focusing on stress, hypoxic, and metabolic adaptations.

Species	Application	Title
	WB	BMC Genomics The fiber diameter traits of Tibetan cashmere goats are governed by the inherent differences in stress, hypoxic, and metabolic adaptations: an integrative study of proteome and transcriptome. Authors - Bingru Zhao View Article