Tested Applications
| Positive WB detected in | A549 cells, HeLa cells, K-562 cells |
| Positive IP detected in | K-562 cells |
| Positive IHC detected in | human lung squamous cell carcinoma tissue, human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
18847-1-AP targets IRF2BP2 in WB, IHC, IF/ICC, IP, CoIP, ChIP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | interferon regulatory factor 2 binding protein 2 |
| Calculated Molecular Weight | 61 kDa |
| Observed Molecular Weight | 65-70 kDa |
| GenBank Accession Number | NM_182972 |
| Gene Symbol | IRF2BP2 |
| Gene ID (NCBI) | 359948 |
| RRID | AB_10598163 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q7Z5L9 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
IRF2BP2 belongs to the IRF2BP family. IRF2BP2 acts as a transcriptional repressor and as a transcriptional corepressor in a IRF2-dependent manner. This repression is not mediated at least in part by histone deacetylase activities.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for IRF2BP2 antibody 18847-1-AP | Download protocol |
| IHC protocol for IRF2BP2 antibody 18847-1-AP | Download protocol |
| IP protocol for IRF2BP2 antibody 18847-1-AP | Download protocol |
| WB protocol for IRF2BP2 antibody 18847-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The IRF2BP2 Polyclonal antibody (Cat# 18847-1-AP) has 17 citations. These appear in journals including Nature Communications, Cancer Discovery, Hepatology, Cell Death & Disease, Hypertension, EMBO Reports, Signal Transduction and Targeted Therapy, PLoS Genetics, Molecular Cell Proteomics, Journal of Experimental Medicine, and others. Research fields span inflammation, cancer (AML, liver, gastric), SUMOylation, cardiac hypertrophy, psoriasis, EGFR signaling, gluconeogenesis, and endothelial EMT.
| Species | Application | Title |
|---|---|---|
Signal Transduct Target Ther Malate initiates a proton-sensing pathway essential for pH regulation of inflammation | ||
Cancer Discov Unleashing Cell-Intrinsic Inflammation as a Strategy to Kill AML Blasts.
| ||
Nat Commun IRF2BP2 binds to a conserved RxSVI motif of protein partners and regulates megakaryocytic differentiation | ||
Hepatology The novel tumor suppressor IRF2BP2 regulates Hippo pathway in liver cancer via a feedback loop. | ||
Cell Death Dis The Sumo proteome of proliferating and neuronal-differentiating cells reveals Utf1 among key Sumo targets involved in neurogenesis. |
