Tested Applications
| Positive WB detected in | rat liver tissue |
| Positive IP detected in | mouse liver tissue |
| Positive IHC detected in | rat liver tissue, human lung cancer tissue, human colon tissue, human kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:12000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
18897-1-AP targets ADH1C in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag13528 Product name: Recombinant human ADH1C protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-374 aa of BC062476 Sequence: MSTAGKVIKCKAAVLWELKKPFSIEEVEVAPPKAHEVRIKMVAAGICRSDEHVVSGNLVTPLPVILGHEAAGIVESVGEGVTTVKPGDKVIPLFTPQCGKCRICKNPESNYCLKNDLGNPRGTLQDGTRRFTCSGKPIHHFVGVSTFSQYTVVDENAVAKIDAASPLEKVCLIGCGFSTGYGSAVKVAKVTPGSTCAVFGLGGVGLSVVMGCKAAGAARIIAVDINKDKFAKAKELGATECINPQDYKKPIQEVLKEMTDGGVDFSFEVIGQLDTMMASLLCCHEACGTSVIVGVPPDSQNLSINPMLLLTGRTWKGAIFGGFKSKESVPKLVADFMAKKFSLDALITNVLPFEKINEGFDLLRSGKSIRTVLT Predict reactive species |
| Full Name | alcohol dehydrogenase 1C (class I), gamma polypeptide |
| Calculated Molecular Weight | 375 aa, 40 kDa |
| Observed Molecular Weight | 37-45 kDa |
| GenBank Accession Number | BC062476 |
| Gene Symbol | ADH1C |
| Gene ID (NCBI) | 126 |
| RRID | AB_10596950 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P00326 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
ADH1C(Alcohol dehydrogenase 1C) is also named as ADH3 and belongs to the zinc-containing alcohol dehydrogenase family.It catalyzes the rate-limiting step for ethanol metabolism: the oxidation of alcohol to acetaldehyde.The ADH1C gene is active in intestine and kidney in fetal and early postnatal life, and persists in the stomach and liver in adult life(PMID:4748759).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for ADH1C antibody 18897-1-AP | Download protocol |
| IHC protocol for ADH1C antibody 18897-1-AP | Download protocol |
| IP protocol for ADH1C antibody 18897-1-AP | Download protocol |
| WB protocol for ADH1C antibody 18897-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
ADH1C Polyclonal antibody (Cat# 18897-1-AP) has 9 citations published in Advanced Science, Journal of Translational Medicine, Free Radical Biology and Medicine, Biochemical Pharmacology, Pharmaceuticals, BMC Gastroenterology, Experimental Eye Research, and Human Mutation. Research fields span antitumor immunity in melanoma, triple negative breast cancer biomarkers, ethanol metabolism, inflammatory responses in ulcerative colitis, adipose atrophy, myocardial ischemia-reperfusion injury, hepatocellular carcinoma progression, neurotrophic keratopathy, and liver cirrhosis.
| Species | Application | Title |
|---|---|---|
Adv Sci (Weinh) Targeting DNGR-1 with Fangchinoline Elevates Dendritic Cell Antigen Cross-Presentation-Mediated Antitumor Immunity in Melanoma | ||
J Transl Med Comprehensive analysis of the metabolomics and transcriptomics uncovers the dysregulated network and potential biomarkers of Triple Negative Breast Cancer | ||
Free Radic Biol Med PPARα agonist WY-14,643 enhances ethanol metabolism in mice: role of catalase. | ||
Biochem Pharmacol PPARα agonist WY-14,643 induces adipose atrophy and fails to blunt chronic ethanol-induced hepatic fat accumulation in mice lacking adipose FGFR1. | ||
Biochem Pharmacol The role of alcohol dehydrogenase 1C in regulating inflammatory responses in ulcerative colitis. | ||
Pharmaceuticals (Basel) Shenqi Granules Enhance Recovery from Myocardial Ischemia-Reperfusion Injury by Downregulating MMP9 and ADH1C. |
