MIS12 Polyclonal antibody

MIS12 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 19820-1-AP

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

hMis12, KNTC2AP, MTW1, Protein MIS12 homolog

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

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Filter: AllWBIHCIF/ICC

WB analysis using 19820-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 19820-1-AP (MIS12 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of mouse kidney using 19820-1-AP
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 19820-1-AP (MIS12 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of mouse ovary using 19820-1-AP
Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 19820-1-AP (MIS12 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of rat ovary using 19820-1-AP
Immunohistochemical analysis of paraffin-embedded rat ovary tissue slide using 19820-1-AP (MIS12 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of rat small intestine using 19820-1-AP
Immunohistochemical analysis of paraffin-embedded rat small intestine tissue slide using 19820-1-AP (MIS12 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human ovary cancer using 19820-1-AP
Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 19820-1-AP (MIS12 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human placenta using 19820-1-AP
Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 19820-1-AP (MIS12 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of mouse ovary using 19820-1-AP
Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 19820-1-AP (MIS12 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of mouse small intestine using 19820-1-AP
Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 19820-1-AP (MIS12 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of rat kidney using 19820-1-AP
Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 19820-1-AP (MIS12 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of rat ovary using 19820-1-AP
Immunohistochemical analysis of paraffin-embedded rat ovary tissue slide using 19820-1-AP (MIS12 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of rat small intestine using 19820-1-AP
Immunohistochemical analysis of paraffin-embedded rat small intestine tissue slide using 19820-1-AP (MIS12 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human ovary cancer using 19820-1-AP
Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 19820-1-AP (MIS12 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human ovary cancer using 19820-1-AP
Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 19820-1-AP (MIS12 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IF Staining of HeLa using 19820-1-AP
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using MIS12 antibody (19820-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Overview

Tested Applications
Product Information
Protocols

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MSDS SDS

Tested Applications

Positive WB detected in	HEK-293 cells, HeLa cells, HepG2 cells
Positive IHC detected in	human ovary cancer tissue, mouse ovary tissue, rat small intestine tissue, human placenta tissue, mouse small intestine tissue, rat kidney tissue, rat ovary tissue, mouse kidney tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:4000
Immunohistochemistry (IHC)	IHC : 1:200-1:800
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

19820-1-AP targets MIS12 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag13870 Product name: Recombinant human MIS12 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-205 aa of BC000229 Sequence: MSVDPMTYEAQFFGFTPQTCMLRIYIAFQDYLFEVMQAVEQVILKKLDGIPDCDISPVQIRKCTEKFLCFMKGHFDNLFSKMEQLFLQLILRIPSNILLPEDKCKETPYSEEDFQHLQKEIEQLQEKYKTELCTKQALLAELEEQKIVQAKLKQTLTFFDELHNVGRDHGTSDFRESLVSLVQNSRKLQNIRDNVEKESKRLKIS Predict reactive species
Full Name	MIS12, MIND kinetochore complex component, homolog (yeast)
Calculated Molecular Weight	205 aa, 24 kDa
Observed Molecular Weight	25-28 kDa
GenBank Accession Number	BC000229
Gene Symbol	MIS12
Gene ID (NCBI)	79003
RRID	AB_3669315
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q9H081
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The MIS12 complex, composed of the Mis12, Pmf1, Nsl1 and Dsn1 subunits, is a highly conserved kinetochore protein that interacts with the Ndc80 and Knl1 complexes, forming the KMN network (PMID: 32341029). MIS12 is a part of the MIS12 complex which is required for normal chromosome alignment and segregation and for kinetochore formation during mitosis. MIS12 is essential for proper kinetochore microtubule attachments (PubMed:23891108)

Protocols

Product Specific Protocols
IF protocol for MIS12 antibody 19820-1-AP	Download protocol
IHC protocol for MIS12 antibody 19820-1-AP	Download protocol
WB protocol for MIS12 antibody 19820-1-AP	Download protocol