Tested Applications
| Positive IHC detected in | mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse brain tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
19955-1-AP targets mGluR1 in IHC, IF-P, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | glutamate receptor, metabotropic 1 |
| Calculated Molecular Weight | 132 kDa |
| GenBank Accession Number | NM_000838 |
| Gene Symbol | GRM1 |
| Gene ID (NCBI) | 2911 |
| RRID | AB_10638312 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q13255 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
GRM1, also named as GPRC1A and MGLUR1, belongs to the G-protein coupled receptor 3 family. GRM1 is a receptor for glutamate. The activity of GRM1 is mediated by a G-protein that activates a phosphatidylinositol-calcium second messenger system. It may participate in the central action of glutamate in the CNS, such as long-term potentiation in the hippocampus and long-term depression in the cerebellum. The antibody can recognize isoform1 and isoform2 of GRM1.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for mGluR1 antibody 19955-1-AP | Download protocol |
| IHC protocol for mGluR1 antibody 19955-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The GRM1/mGluR1 polyclonal antibody (Cat# 19955-1-AP) has 4 citations published in Int J Biol Sci, Clin Cancer Res, J Transl Med, and Brain Commun. Research fields span glutamate signaling in neurological disorders, prostate cancer biology, tumor immunology (CD8⁺ T cell exhaustion in oral squamous cell carcinoma), and Alzheimer's disease pathology.
| Species | Application | Title |
|---|---|---|
Int J Biol Sci Glutamate signaling in benign and malignant disorders: current status, future perspectives, and therapeutic implications. | ||
Clin Cancer Res Serum glutamate levels correlate with Gleason score and glutamate blockade decreases proliferation, migration, and invasion and induces apoptosis in prostate cancer cells. | ||
J Transl Med ALDH1L1 reverses CD8(+) T cell exhaustion in the oral squamous cell carcinoma microenvironment by reprogramming L-glutamate metabolism. | ||
Brain Commun M1 muscarinic acetylcholine receptor dysfunction in moderate Alzheimer's disease pathology. |
