Tested Applications
| Positive WB detected in | A549 cells, T-47D cells, HEK-293 cells, MCF-7 cells |
| Positive IF-P detected in | mouse liver tissue |
| Positive IF/ICC detected in | MCF-7 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
20057-1-AP targets CDH26 in WB, IF/ICC, IF-P, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | cadherin-like 26 |
| Calculated Molecular Weight | 95 kDa |
| Observed Molecular Weight | 18-22 kDa, 95-100 kDa |
| GenBank Accession Number | NM_021810 |
| Gene Symbol | CDH26 |
| Gene ID (NCBI) | 60437 |
| RRID | AB_2878637 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q8IXH8 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
CDH26, also named VR20, is a member of cadherins which are calcium-dependent cell adhesion proteins. Cadherins preferentially interact with themselves in a homophilic manner in connecting cells. Cadherins may thus contribute to the sorting of heterogeneous cell types. CDH26 produces 4 isoforms through alternative splicing, with molecular weights of 13, 18, 92, and 95 kDa, respectively.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CDH26 antibody 20057-1-AP | Download protocol |
| WB protocol for CDH26 antibody 20057-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The CDH26 Polyclonal antibody (catalog number 20057-1-AP) has 1 total citation. It appears in the journal Cell Discovery, in a study titled "Cadherin-26 (CDH26) regulates airway epithelial cell cytoskeletal structure and polarity." The research field involves airway epithelial biology, specifically cytoskeletal organization and cell polarity. The antibody was applied in Western blot (WB) and immunofluorescence (IF) assays using human samples.
| Species | Application | Title |
|---|---|---|
Cell Discov Cadherin-26 (CDH26) regulates airway epithelial cell cytoskeletal structure and polarity.
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