Tested Applications
| Positive WB detected in | HEK-293 cells, COLO 320 cells, HeLa cells, HepG2 cells |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:10-1:100 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
20080-1-AP targets MAS1L in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | MAS1 oncogene-like |
| Calculated Molecular Weight | 42 kDa |
| Observed Molecular Weight | 42 kDa |
| GenBank Accession Number | NM_052967 |
| Gene Symbol | MAS1L |
| Gene ID (NCBI) | 116511 |
| RRID | AB_10665372 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P35410 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
MAS1L, also named MRG, belongs to the G-protein coupled receptor 1 family and Mas subfamily. The antibody is specific to MAS1L.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for MAS1L antibody 20080-1-AP | Download protocol |
| WB protocol for MAS1L antibody 20080-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The ACE2 antibody (Cat# 20080-1-AP) has 29 total citations published in journals including Redox Biology, Journal of Translational Medicine, British Journal of Pharmacology, Journal of Ethnopharmacology, International Immunopharmacology, ACS Omega, and Journal of Molecular and Cellular Cardiology. Associated research fields span sepsis and inflammation, cardiovascular disease (hypertension, cardiomyopathy), the renin-angiotensin system, pulmonary fibrosis, acute lung injury, neurological disorders, gut barrier dysfunction, and atherosclerosis.
| Species | Application | Title |
|---|---|---|
Redox Biol Myeloid ACE2 protects against septic hypotension and vascular dysfunction through Ang-(1-7)-Mas-mediated macrophage polarization | ||
J Transl Med ApoE4 associated with severe COVID-19 outcomes via downregulation of ACE2 and imbalanced RAS pathway | ||
J Transl Med Mas receptor activation regulates the functional phenotype of myocardial macrophages through the Akt/Nrf2 signaling pathway to alleviate sepsis-induced cardiomyopathy. | ||
Phytother Res α-Mangostin Attenuates Blood Pressure and Reverses Vascular Remodeling by Balancing ACE/AT1R and ACE2/Ang-(1-7)/MasR Axes in Ang II-Infused Hypertensive Mice | ||
Br J Pharmacol Angiotensin-(1-7) alleviates intestinal barrier dysfunction and dysbiosis in mice with polymicrobial sepsis. | ||
J Ethnopharmacol Synergistic effect of constituent drugs of Baibutang on improving Yin-deficiency pulmonary fibrosis in rats |
