Tested Applications
| Positive WB detected in | HeLa cells, HEK-293 cells, LoVo cells |
| Positive IHC detected in | human kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
20715-1-AP targets GHRHR in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | GHRH receptor |
| Calculated Molecular Weight | 47 kDa |
| Observed Molecular Weight | 48 kDa |
| GenBank Accession Number | NM_000823 |
| Gene Symbol | GHRHR |
| Gene ID (NCBI) | 2692 |
| RRID | AB_11232423 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q02643 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
GHRHR, also named GRFR, belongs to the G-protein coupled receptor 2 family. It is a GRF receptor coupled to G proteins that activate adenylyl cyclase. GHRHR stimulates somatotroph cell growth, GH gene transcription, and GH secretion. The antibody is specific to GHRHR. The antibody is specific to GHRHR.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for GHRHR antibody 20715-1-AP | Download protocol |
| WB protocol for GHRHR antibody 20715-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The GHRHR Polyclonal antibody (catalog number 20715-1-AP) has a total of 1 citation. This citation appears in the journal Neural Regen Res (Neural Regeneration Research), published in 2023. The associated research field is neural regeneration, specifically examining how growth hormone promotes reconstruction of injured axons in the hypothalamo-neurohypophyseal system. The antibody was applied in immunofluorescence (IF) using mouse samples.
| Species | Application | Title |
|---|---|---|
Neural Regen Res Growth hormone promotes the reconstruction of injured axons in the hypothalamo-neurohypophyseal system |
