Tested Applications
| Positive WB detected in | Y79 cells, SH-SY5Y cells, HEK-293 cells |
| Positive IP detected in | SH-SY5Y cells |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
20766-1-AP targets CAPRIN2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag14745 Product name: Recombinant human CAPRIN2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 970-1128 aa of BC007520 Sequence: MTPVDVPVTNPAATILPVHVYPLPQQMRVAFSAARTSNLAPGTLDQPIVFDLLLNNLGETFDLQLGRFNCPVNGTYVFIFHMLKLAVNVPLYVNLMKNEEVLVSAYANDGAPDHETASNHAILQLFQGDQIWLRLHRGAIYGSSWKYSTFSGYLLYQD Predict reactive species |
| Full Name | caprin family member 2 |
| Calculated Molecular Weight | 1127 aa, 126 kDa |
| Observed Molecular Weight | 126-150 kDa |
| GenBank Accession Number | BC007520 |
| Gene Symbol | CAPRIN2 |
| Gene ID (NCBI) | 65981 |
| RRID | AB_10695763 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q6IMN6 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CAPRIN2 antibody 20766-1-AP | Download protocol |
| IHC protocol for CAPRIN2 antibody 20766-1-AP | Download protocol |
| IP protocol for CAPRIN2 antibody 20766-1-AP | Download protocol |
| WB protocol for CAPRIN2 antibody 20766-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The CAPRIN2 Polyclonal antibody (Cat# 20766-1-AP) has 2 citations. One appears in Neuroendocrinology (2022), studying transcriptional and post-transcriptional regulation of oxytocin and vasopressin gene expression in rat. The other is in Developmental Dynamics (2015), investigating Caprin2 deficiency causing lens defects and Peters anomaly features in mouse. The associated research fields are neuroendocrinology and developmental biology.
| Species | Application | Title |
|---|---|---|
Neuroendocrinology Transcriptional and post-transcriptional regulation of oxytocin and vasopressin gene expression by CREB3L1 and CAPRIN2. | ||
Dev Dyn Deficiency of the RNA binding protein Caprin2 causes lens defects and features of Peters anomaly.
|
Reviews
What customers say
The two reviews reflect mixed performance. One user (4/5) reported the antibody worked well for Western Blot and Immunoprecipitation on HEK293T cells, as well as WB and IF on rat tissue, but noted it failed for IP on rat neuronal samples. The other user (2/5) tested it on MRC-9 lung fibroblasts by WB and could not detect Caprin2, suggesting either poor detection or absence of the target in that cell line. Overall, the antibody shows reliable performance in certain cell types and tissues but may have limitations depending on the sample and application.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Paul (Verified Customer) (04-29-2019) | Antibody works very well for WB and IP from HEK293T cells, and works well for WB and IF from rat tissue samples. However, I couldn't get the antibody to work for IP from rat neuronal samples.
 |
Criseyda (Verified Customer) (09-21-2018) | Two possibilities: Antibody is not detecting Carping protein well or Caprin2 is not expressed in these cells.
 |
