Tested Applications
| Positive WB detected in | A549 cells, HeLa cells, Jurkat cells |
| Positive IP detected in | Jurkat cells |
| Positive IHC detected in | human colon tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:100-1:400 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
21146-1-AP targets LARS in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag15684 Product name: Recombinant human LARS protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 827-1176 aa of BC151214 Sequence: EFQAAKDKYRELAVEGMHRELVFRFIEVQTLLLAPFCPHLCEHIWTLLGKPDSIMNASWPVAGPVNEVLIHSSQYLMEVTHDLRLRLKNYMMPAKGKKTDKQPLQKPSHCTIYVAKNYPPWQHTTLSVLRKHFEANNGKLPDNKVIASELGSMPELKKYMKKVMPFVAMIKENLEKMGPRILDLQLEFDEKAVLMENIVYLTNSLELEHIEVKFASEAEDKIREDCCPGKPLNVFRIEPGVSVSLVNPQPSNGHFSTKIEIRQGDNCDSIIRRLMKMNRGIKDLSKVKLMRFDDPLLGPRRVPVLGKEYTEKTPISEHAVFNVDLMSKKIHLTENGIRVDIGDTIIYLVH Predict reactive species |
| Full Name | leucyl-tRNA synthetase |
| Calculated Molecular Weight | 1176 aa, 134 kDa |
| Observed Molecular Weight | 135-140 kDa |
| GenBank Accession Number | BC151214 |
| Gene Symbol | LARS |
| Gene ID (NCBI) | 51520 |
| RRID | AB_10733878 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9P2J5 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for LARS antibody 21146-1-AP | Download protocol |
| IHC protocol for LARS antibody 21146-1-AP | Download protocol |
| IP protocol for LARS antibody 21146-1-AP | Download protocol |
| WB protocol for LARS antibody 21146-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The LARS Polyclonal antibody (Cat# 21146-1-AP) has 19 citations. Journals include Cell Stem Cell, Nature Cell Biology, Autophagy, Nature Communications, Redox Biology, Molecular Cell, Journal of Experimental & Clinical Cancer Research, Advanced Science, Journal of Translational Medicine, PNAS, European Journal of Pharmaceutical Sciences, Cancers, Cell Signaling, Journal of Cancer, Frontiers in Oncology, Biomedical Research International, PLoS One, Human Cell, and International Journal of General Medicine. Research fields span leukemia, mTORC1 signaling, tRNA aminoacylation, osteosarcoma, ovarian cancer, hepatocellular carcinoma, colorectal cancer, diabetic kidney disease, and ferroptosis.
| Species | Application | Title |
|---|---|---|
Cell Stem Cell METTL16 drives leukemogenesis and leukemia stem cell self-renewal by reprogramming BCAA metabolism | ||
Autophagy KAT7-mediated CANX (calnexin) crotonylation regulates leucine-stimulated MTORC1 activity. | ||
Nat Commun Phenylalanine-tRNA aminoacylation is compromised by ALS/FTD-associated C9orf72 C4G2 repeat RNA | ||
Redox Biol Caloric restriction exacerbates renal post-ischemic injury and fibrosis by modulating mTORC1 signaling and autophagy | ||
Mol Cell A methyltransferase-independent role for METTL1 in tRNA aminoacylation and oncogenic transformation |
