Tested Applications
| Positive WB detected in | human plasma, human serum |
| Positive IP detected in | human plasma tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
21247-1-AP targets ITIH3 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag15750 Product name: Recombinant human ITIH3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 601-782 aa of BC107604 Sequence: MVVTKPEDNEDERAIADKPGEDAEATPVSPAMSYLTSYQPPQNPYYYVDGDPHFIIQIPEKDDALCFNIDEAPGTVLRLIQDAVTGLTVNGQITGDKRGSPDSKTRKTYFGKLGIANAQMDFQVEVTTEKITLWNRAVPSTFSWLDTVTVTQDGLSMMINRKNMVVSFGDGVTFVVVLHQVW Predict reactive species |
| Full Name | inter-alpha (globulin) inhibitor H3 |
| Calculated Molecular Weight | 890 aa, 100 kDa |
| Observed Molecular Weight | 100 kDa |
| GenBank Accession Number | BC107604 |
| Gene Symbol | ITIH3 |
| Gene ID (NCBI) | 3699 |
| RRID | AB_2878830 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q06033 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
ITIH3, or inter-alpha-trypsin inhibitor heavy chain 3, is a gene in humans that encodes the heavy chain subunit of the pre-alpha-trypsin inhibitor complex. This complex plays a role in stabilizing the extracellular matrix through its ability to bind hyaluronic acid. The gene is located on chromosome 3 and is part of the inter-alpha-trypsin inhibitor family gene cluster. ITIH3 has restricted expression primarily toward the liver, with an RPKM value of 228.8, indicating a relatively high level of expression in this organ.
Protocols
| Product Specific Protocols | |
|---|---|
| IP protocol for ITIH3 antibody 21247-1-AP | Download protocol |
| WB protocol for ITIH3 antibody 21247-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The ITIH3 Polyclonal antibody (Cat# 21247-1-AP) has 6 total citations published in Br J Cancer, Sci Rep, Mol Oncol, J Pers Med, Dis Markers, and Arch Biochem Biophys. Research fields span pancreatic cancer biomarker discovery, endometrial cancer prognostic signatures, colorectal cancer diagnostics, dairy cattle plasma protein analysis, and diabetic nephropathy mechanisms involving oxidative stress and autophagy regulation.
| Species | Application | Title |
|---|---|---|
Br J Cancer A new panel of pancreatic cancer biomarkers discovered using a mass spectrometry-based pipeline. | ||
Mol Oncol Identification of serum protein biomarkers for pre-cancerous lesions associated with pancreatic ductal adenocarcinoma. | ||
J Pers Med Comprehensive Analysis and Experimental Validation of a Novel Estrogen/Progesterone-Related Prognostic Signature for Endometrial Cancer. | ||
Dis Markers Plasma Inter-Alpha-Trypsin Inhibitor Heavy Chains H3 and H4 Serve as Novel Diagnostic Biomarkers in Human Colorectal Cancer. | ||
Arch Biochem Biophys Targeting ITIH3 inhibits oxidative stress and autophagy in renal tubular epithelial cells by regulating the HSP90B1/STAT3 axis, thereby alleviating diabetic nephropathy injury. |
