Tested Applications
| Positive WB detected in | A375 cells |
| Positive IHC detected in | human tonsillitis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | human tonsillitis tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
22228-1-AP targets FOXP3 in WB, IHC, IF-P, IP, CoIP, ChIP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse, rat, pig, chicken |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag17571 Product name: Recombinant human FOXP3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-330 aa of BC113401 Sequence: MPNPRPGKPSAPSLALGPSPGASPSWRAAPKASDLLGARGPGGTFQGRDLRGGAHASSSSLNPMPPSQLQLPTLPLVMVAPSGARLGPLPHLQALLQDRPHFMHQLSTVDAHARTPVLQVHPLESPAMISLTPPTTATGVFSLKARPGLPPGINVASLEWVSREPALLCTFPNPSAPRKDSTLSAVPQSSYPLLANGVCKWPGCEKVFEEPEDFLKHCQADHLLDEKGRAQCLLQREMVQSLEQQLVLEKEKLSAMQAHLAGKMALTKASSVASSDKGSCCIVAAGSQGPVVPAWSGPREAPDSLFAVRRHLWGSHGNSTFPEFLHNMDY Predict reactive species |
| Full Name | forkhead box P3 |
| Calculated Molecular Weight | 431 aa, 47 kDa |
| Observed Molecular Weight | 50-55 kDa |
| GenBank Accession Number | BC113401 |
| Gene Symbol | FOXP3 |
| Gene ID (NCBI) | 50943 |
| RRID | AB_11182376 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9BZS1 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
FOXP3, also named as IPEX, JM2 and Scurfin, is a transcription factor. FOXP3 plays a critical role in the control of immune response. Defects in FOXP3 are the cause of immunodeficiency polyendocrinopathy, enteropathy, X-linked syndrome (IPEX) which also known as X-linked autoimmunity-immunodeficiency syndrome. This antibody is a rabbit polyclonal antibody raised against a peptide mapping within human FOXP3.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for FOXP3 antibody 22228-1-AP | Download protocol |
| IHC protocol for FOXP3 antibody 22228-1-AP | Download protocol |
| WB protocol for FOXP3 antibody 22228-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
22228-1-AP is a FOXP3 affinity-purified antibody with 133 total citations. Citations appear in journals including Cell Mol Immunol, Cancer Res, ACS Nano, Theranostics, Biomaterials, Cancer Lett, Cell Death Dis, Front Immunol, J Exp Clin Cancer Res, Adv Sci, Int Immunopharmacol, Phytomedicine, and J Transl Med, among many others. Research fields span immunology, oncology, inflammatory bowel disease, autoimmune disorders, the microbiome–gut axis, and Treg/Th17 immune regulation.
| Species | Application | Title |
|---|---|---|
Cell Mol Immunol rFliC prolongs allograft survival in association with the activation of recipient Tregs in a TLR5-dependent manner. | ||
Cancer Res Epigenetic regulation of CXCL12 plays a critical role in mediating tumor progression and the immune response in osteosarcoma. | ||
ACS Nano Remodeling of Effector and Regulatory T Cells by Capture and Utilization of miRNAs Using Nanocomposite Hydrogel for Tumor-Specific Photothermal Immunotherapy | ||
Theranostics Epigenetically regulated lncRNAs dissect the intratumoural heterogeneity and facilitate immune evasion of glioblastomas | ||
Genes Dis SIGLEC15 modulates the immunosuppressive microenvironment and suppresses malignant phenotypes in triple-negative breast cancer. | ||
Acta Pharm Sin B Self-assembled FeS-based cascade bioreactor with enhanced tumor penetration and synergistic treatments to trigger robust cancer immunotherapy |
Reviews
What customers say
The single review for this product reports a 2-star rating from an Immunofluorescence application on mouse brain slices at a 1:100 dilution. The reviewer noted non-specific staining as the main issue, suggesting the antibody may require optimization (e.g., adjusting dilution, blocking conditions, or fixation protocol) to improve specificity in IF experiments.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Anne-Laure (Verified Customer) (12-07-2021) | non-specific staining
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