MPEG1 Polyclonal antibody

MPEG1 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 23960-1-AP

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

macrophage expressed 1, Macrophage gene 1 protein, Macrophage-expressed gene 1 protein, Macrophage-expressed gene 1 protein, processed form, Mpg 1

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

SKU:

-/ -

Freight/Packing: -

Quantity

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Filter: AllWBIHC

WB analysis of HeLa using 23960-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 23960-1-AP (MPEG1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis using 23960-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 23960-1-AP (MPEG1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of HeLa using 23960-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 23960-1-AP (MPEG1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human rectal cancer using 23960-1-AP
Immunohistochemical analysis of paraffin-embedded human rectal cancer tissue slide using 23960-1-AP (MPEG1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human rectal cancer using 23960-1-AP
Immunohistochemical analysis of paraffin-embedded human rectal cancer tissue slide using 23960-1-AP (MPEG1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of rat spleen using 23960-1-AP
Immunohistochemical analysis of paraffin-embedded rat spleen tissue slide using 23960-1-AP (MPEG1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of rat spleen using 23960-1-AP
Immunohistochemical analysis of paraffin-embedded rat spleen tissue slide using 23960-1-AP (MPEG1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of rat brain using 23960-1-AP
Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 23960-1-AP (MPEG1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

Overview

Tested Applications
Product Information
Protocols

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MSDS SDS

Tested Applications

Positive WB detected in	HeLa cells, HepG2 cells, RAW 264.7 cells
Positive IHC detected in	human rectal cancer tissue, rat brain tissue, rat spleen tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunohistochemistry (IHC)	IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

23960-1-AP targets MPEG1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag21055 Product name: Recombinant human MPEG1 protein Source: e coli.-derived, PET30a Tag: 6*His Domain: 25-370 aa of BC104997 Sequence: EMDEVGVQKCKNALKLPVLEVLPGGGWDNLRNVDMGRVMELTYSNCRTTEDGQYIIPDEIFTIPQKQSNLEMNSEILESWANYQSSTSYSINTELSLFSKVNGKFSTEFQRMKTLQVKDQAITTRVQVRNLVYTVKINPTLELSSGFRKELLDISDRLENNQTRMATYLAELLVLNYGTHVTTSVDAGAALIQEDHLRASFLQDSQSSRSAVTASAGLAFQNTVNFKFEENYTSQNVLTKSYLSNRTNSRVQSIGGVPFYPGITLQAWQQGITNHLVAIDRSGLPLHFFINPNMLPDLPGPLVKKVSKTVETAVKRYYTFNTYPGCTDLNSPNFNFQANTDDGSCE Predict reactive species
Full Name	macrophage expressed 1
Calculated Molecular Weight	716 aa, 79 kDa
Observed Molecular Weight	70 kDa
GenBank Accession Number	BC104997
Gene Symbol	MPEG1
Gene ID (NCBI)	219972
RRID	AB_2879376
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen Affinity purified
UNIPROT ID	Q2M385
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MPEG1 (macrophage-expressed gene 1), also known as perforin-2, is a pore-forming protein of the membrane attack complex/perforin (MACPF) superfamily. Predominantly expressed in macrophages and leukocytes, it is upregulated by proinflammatory signals such as TNF-α and LPS. MPEG1 localizes to phagosomes and lysosomes, where it breaches bacterial membranes to facilitate antimicrobial defense and antigen cross-presentation. (PMID: 26402460)