MPEG1 Polyclonal antibody, PBS Only

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• MPEG1 Polyclonal antibody, PBS Only (23960-1-PBS)

MPEG1 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 23960-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

macrophage expressed 1, Macrophage gene 1 protein, Macrophage-expressed gene 1 protein, Macrophage-expressed gene 1 protein, processed form, Mpg 1

• 

  WB analysis of HeLa using 23960-1-AP (same clone as 23960-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 23960-1-AP (MPEG1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 23960-1-PBS in a different storage buffer formulation.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis using 23960-1-AP (same clone as 23960-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 23960-1-AP (MPEG1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 23960-1-PBS in a different storage buffer formulation.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of HeLa using 23960-1-AP (same clone as 23960-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 23960-1-AP (MPEG1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 23960-1-PBS in a different storage buffer formulation.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human rectal cancer using 23960-1-AP (same clone as 23960-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human rectal cancer tissue slide using 23960-1-AP (MPEG1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 23960-1-PBS in a different storage buffer formulation.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human rectal cancer using 23960-1-AP (same clone as 23960-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human rectal cancer tissue slide using 23960-1-AP (MPEG1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 23960-1-PBS in a different storage buffer formulation.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of rat spleen using 23960-1-AP (same clone as 23960-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat spleen tissue slide using 23960-1-AP (MPEG1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 23960-1-PBS in a different storage buffer formulation.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of rat spleen using 23960-1-AP (same clone as 23960-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat spleen tissue slide using 23960-1-AP (MPEG1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 23960-1-PBS in a different storage buffer formulation.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of rat brain using 23960-1-AP (same clone as 23960-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 23960-1-AP (MPEG1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 23960-1-PBS in a different storage buffer formulation.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Product Information

23960-1-PBS targets MPEG1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag21055

  Product name: Recombinant human MPEG1 protein

  Source: e coli.-derived, PET30a

  Tag: 6*His

  Domain: 25-370 aa of BC104997

  Sequence: EMDEVGVQKCKNALKLPVLEVLPGGGWDNLRNVDMGRVMELTYSNCRTTEDGQYIIPDEIFTIPQKQSNLEMNSEILESWANYQSSTSYSINTELSLFSKVNGKFSTEFQRMKTLQVKDQAITTRVQVRNLVYTVKINPTLELSSGFRKELLDISDRLENNQTRMATYLAELLVLNYGTHVTTSVDAGAALIQEDHLRASFLQDSQSSRSAVTASAGLAFQNTVNFKFEENYTSQNVLTKSYLSNRTNSRVQSIGGVPFYPGITLQAWQQGITNHLVAIDRSGLPLHFFINPNMLPDLPGPLVKKVSKTVETAVKRYYTFNTYPGCTDLNSPNFNFQANTDDGSCE

• Full Name: macrophage expressed 1
• Calculated Molecular Weight: 716 aa, 79 kDa
• Observed Molecular Weight: 70 kDa
• GenBank Accession Number: BC104997
• Gene Symbol: MPEG1
• Gene ID (NCBI): 219972
• RRID: AB_2879376
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen Affinity purified
• UNIPROT ID: Q2M385
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

MPEG1 (macrophage-expressed gene 1), also known as perforin-2, is a pore-forming protein of the membrane attack complex/perforin (MACPF) superfamily. Predominantly expressed in macrophages and leukocytes, it is upregulated by proinflammatory signals such as TNF-α and LPS. MPEG1 localizes to phagosomes and lysosomes, where it breaches bacterial membranes to facilitate antimicrobial defense and antigen cross-presentation. (PMID: 26402460)