Tested Applications
| Positive WB detected in | mouse testis tissue, NIH/3T3 cells, Jurkat cells |
| Positive IHC detected in | human stomach tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | NIH/3T3 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
24896-1-AP targets CCT2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag19264 Product name: Recombinant human CCT2 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 186-535 aa of BC113516 Sequence: AVLRLKGSGNLEAIHIIKKLGGSLADSYLDEGFLLDKKIGVNQPKRIENAKILIANTGMDTDKIKIFGSRVRVDSTAKVAEIEHAEKEKMKEKVERILKHGINCFINRQLIYNYPEQLFGAAGVMAIEHADFAGVERLALVTGGEIASTFDHPELVKLGSCKLIEEVMIGEDKLIHFSGVALGEACTIVLRGATQQILDEAERSLHDALCVLAQTVKDSRTVYGGGCSEMLMAHAVTQLANRTPGKEAVAMESYAKALRMLPTIIADNAGYDSADLVAQLRAAHSEGNTTAGLDMREGTIGDMAILGITESFQVKRQVLLSAAEAAEVILRVDNIIKAAPRKRVPDHHPC Predict reactive species |
| Full Name | chaperonin containing TCP1, subunit 2 (beta) |
| Calculated Molecular Weight | 535 aa, 57 kDa |
| Observed Molecular Weight | 50-58 kDa |
| GenBank Accession Number | BC113516 |
| Gene Symbol | CCT2 |
| Gene ID (NCBI) | 10576 |
| RRID | AB_2879783 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P78371 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
CCT2 is a gene encoding a molecular chaperone that is a member of the chaperonin-containing TCP1 complex (CCT), also known as the TCP1 ring complex (TRiC). The switch of CCT2ʹs role from a chaperonin to a specific aggrephagy receptor is achieved by CCT2 monomer formation. CCT2 functions independently of ubiquitin and the TRiC complex to facilitate the autophagic clearance of solid protein aggregates. CCT2 has 2 isoforms with a molecular mass of 53 and 57 kDa. (PMID: 35699934)
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CCT2 antibody 24896-1-AP | Download protocol |
| IHC protocol for CCT2 antibody 24896-1-AP | Download protocol |
| WB protocol for CCT2 antibody 24896-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
Anti-CCT2 antibody (Cat# 24896-1-AP) has 15 citations across 13 journals, including Nature Communications, Molecular Cell, Autophagy, Cell Death & Differentiation, and Signal Transduction and Targeted Therapy. Research fields span autophagy, ferroptosis, cilia biology, cancer (NSCLC, HCC), NAFLD, viral infection, angiogenesis, neuropathic pain, pulmonary fibrosis, and endometriosis. Applications include WB, IF, IP, and IHC in human and mouse models.
| Species | Application | Title |
|---|---|---|
Signal Transduct Target Ther Albumosomes formed by cytoplasmic pre-folding albumin maintain mitochondrial homeostasis and inhibit nonalcoholic fatty liver disease | ||
Autophagy Lassa virus Z protein hijacks the autophagy machinery for efficient transportation by interrupting CCT2-mediated cytoskeleton network formation | ||
Nat Commun Episodic transport of protein aggregates achieves a positive size selectivity in aggresome formation | ||
Reviews
What customers say
All four reviews are 5-star. Users consistently report clear, well-defined bands in Western Blot at dilutions of 1:1000 to 1:2000, with testing in HEK293T and AML cell lines. One reviewer also found the antibody promising for Immunofluorescence. Overall feedback describes the product as performing as expected and highly recommended for routine WB applications.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Loïc (Verified Customer) (01-27-2026) | Good for western blot Seems good for Immunofluorescence as well
 |
Ziwei (Verified Customer) (01-04-2026) | works as it should be.
|
Rashmi (Verified Customer) (02-14-2025) | Used for Western Blot, Bands are clearly visible.
|
Rashmi (Verified Customer) (02-04-2025) | Used for Western blot, Highly Recommended
|
