Tested Applications
| Positive WB detected in | HL-60 cells, HeLa cells, HepG2 cells, Jurkat cells, mouse testis tissue, rat testis tissue |
| Positive IP detected in | Jurkat cells |
| Positive IHC detected in | human ovary cancer tissue, human liver tissue, human ovary tissue, human testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
25349-1-AP targets SENP1 in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag18028 Product name: Recombinant human SENP1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-331 aa of BC045639 Sequence: MDDIADRMRMDAGEVTLVNHNSVFKTHLLPQTGFPEDQLSLSDQQILSSRQGHLDRSFTCSTRSAAYNPSYYSDNPSSDSFLGSGDLRTFGQSANGQWRNSTPSSSSSLQKSRNSRSLYLETRKTSSGLSNSFAGKSNHHCHVSAYEKSFPIKPVPSPSWSGSCRRSLLSPKKTQRRHVSTAEETVQEEEREVYRQLLQMVTGKQFTIAKPTTHFPLHLSRCLSSSKNTLKDSLFKNGNSCASQIIGSDTSSSGSASILTNQEQLSHSVYSLSSYTPDVAFGSKDSGTLHHPHHHHSVPHQPDNLAASNTQSEGSDSVILLKVKDSQTPTP Predict reactive species |
| Full Name | SUMO1/sentrin specific peptidase 1 |
| Calculated Molecular Weight | 644 aa, 73 kDa |
| Observed Molecular Weight | 73-74 kDa |
| GenBank Accession Number | BC045639 |
| Gene Symbol | SENP1 |
| Gene ID (NCBI) | 29843 |
| RRID | AB_2880039 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9P0U3 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
SENP1, Sentrin-specific protease 1, is a cysteine protease that specifically targets members of the small ubiquitin-like modifier (SUMO) protein family. SENP1 catalyzes two functions in the SUMO pathway (PMID: 29506078).The first is the hydrolysis of an alpha-linked peptide bond at the C-terminal end of the small ubiquitin-like modifier (SUMO) propeptides. The second is the deconjugation of SUMO1, SUMO2 and SUMO3 from targeted proteins (PMID: 16253240). SENP1 is located in the nucleus and cytosol.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for SENP1 antibody 25349-1-AP | Download protocol |
| IP protocol for SENP1 antibody 25349-1-AP | Download protocol |
| WB protocol for SENP1 antibody 25349-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The SENP1 antibody (catalog 25349-1-AP) has 24 citations. These appear in journals including Nature, Nature Communications, Autophagy, Cell Reports, Free Radical Biology and Medicine, International Journal of Biological Sciences, iScience, Journal of Virology, Oncology Reports, Journal of Cellular and Molecular Medicine, and others. Research fields span SUMOylation/deSUMOylation biology, autophagy, mitochondrial homeostasis, oxidative stress, ferroptosis, pyroptosis, viral replication, fibrosis, and multiple cancers (liver, colorectal, head and neck, prostate). Applications are predominantly Western blot, with IHC, IF, and Co-IP.
| Species | Application | Title |
|---|---|---|
Nature Lactate regulates cell cycle by remodeling the anaphase promoting complex
| ||
Autophagy A fine-tuning mechanism underlying self-control for autophagy: deSUMOylation of BECN1 by SENP3 | ||
Nat Commun Human ANP32A/B are SUMOylated and utilized by avian influenza virus NS2 protein to overcome species-specific restriction | ||
Int J Biol Sci SENP1 Interacts with HIF1α to Regulate Glycolysis of Prostatic Carcinoma Cells.
| ||
Phytomedicine Bushentongluo formula counteracts BMSCs senescence to alleviate postmenopausal osteoporosis by activating mitophagy via the SENP1-SIRT3 pathway. | ||
Int J Mol Med SENP1 promotes p27kip1 nuclear export though enhanced SUMOylation in cholangiocarcinoma leading to increased cell proliferation and chemoresistance |
Reviews
What customers say
The product has one review. The user reported using it for Western Blot at a 1:1000 dilution on HeLa cells, describing it as a great antibody for immunoblotting. The review was rated 4 out of 5 stars.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Chun (Verified Customer) (09-07-2020) | This is a great antibody for immunoblotting.
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