Tested Applications
| Positive WB detected in | mouse lung tissue, mouse placenta tissue, mouse spleen tissue |
| Positive IF/ICC detected in | SH-SY5Y cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
26533-1-AP targets Ephrin B2 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse, hamster |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag24865 Product name: Recombinant human EFNB2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 168-235 aa of BC074856 Sequence: ASSAGSTRNKDPTRRPELEAGTNGRSSTTSPFVKPNPGSSTDGNSAGHSGNNILGSEVALFAGIASGC Predict reactive species |
| Full Name | ephrin-B2 |
| Calculated Molecular Weight | 333 aa, 37 kDa |
| Observed Molecular Weight | 47-50 kDa |
| GenBank Accession Number | BC074856 |
| Gene Symbol | EFNB2 |
| Gene ID (NCBI) | 1948 |
| RRID | AB_3085878 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P52799 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Ephrin B2 (EFNB2) is a transmembrane ligand for the Ephrin B class (EPHB) of receptor tyrosine kinases. EPHB receptor(EPHB1-EPHB4, EPH6) are located at the member of one cell and activated by binding EFNB protein located on another cell, resulting in a bidirectional Ephrin-EphR signaling (PMID: 26790531). Ephrin-B are involved in vasculogenesis, neural development and synaptic plasticity, and in pathological processes such as cancer or neurodegeneration (PMID:29508392). The calculated molecular weight of EFNB2 is 37 kDa, and there is also detected about 50 kDa band in the article (PMID: 27650867, PMID: 23492730).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for Ephrin B2 antibody 26533-1-AP | Download protocol |
| WB protocol for Ephrin B2 antibody 26533-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The Ephrin B2 Polyclonal antibody (Cat# 26533-1-AP) has 5 citations published in Science Advances, Oncology Reports, Cell Signaling, Viruses, and PLoS Neglected Tropical Diseases. Research fields span neuroscience (anesthesia emergence), oncology (cholangiocarcinoma immune microenvironment), vascular biology (subretinal fibrosis), and virology (cedar virus and henipavirus entry mechanisms).
| Species | Application | Title |
|---|---|---|
Sci Adv EphB1-NR2B receptor signaling in glutamatergic neurons of the ventroposteromedial thalamic nucleus regulates emergence from anesthesia. | ||
Cell Signal siRASA1 in vascular endothelial cells alleviates subretinal fibrosis through inhibiting macrophage-myofibroblast transdifferentiation via SHH signaling pathway. | ||
Oncol Rep Exploring the role of disulfidptosis‑related signatures in immune microenvironment, prognosis and therapeutic strategies of cholangiocarcinoma.
| ||
Viruses Ephrin B1 and B2 Mediate Cedar Virus Entry into Egyptian Fruit Bat Cells
| ||
PLoS Negl Trop Dis Ephrin B2 and Ephrin B3 are receptors for a novel putative henipavirus with zoonotic potential. |
