Tested Applications
| Positive IHC detected in | mouse eye tissue, mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse eye tissue |
| Positive IF/ICC detected in | SH-SY5Y cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
26598-1-AP targets CRB1 in WB, IHC, IF/ICC, IF-P, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag24512 Product name: Recombinant human CRB1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 30-188 aa of BC136271 Sequence: NNTRCLSNSCQNNSTCKDFSKDNDCSCSDTANNLDKDCDNMKDPCFSNPCQGSATCVNTPGERSFLCKCPPGYSGTICETTIGSCGKNSCQHGGICHQDPIYPVCICPAGYAGRFCEIDHDECASSPCQNGAVCQDGIDGYSCFCVPGYQGRHCDLEVD Predict reactive species |
| Full Name | crumbs homolog 1 (Drosophila) |
| Calculated Molecular Weight | 1406 aa, 154 kDa |
| Observed Molecular Weight | 150 kDa |
| GenBank Accession Number | BC136271 |
| Gene Symbol | CRB1 |
| Gene ID (NCBI) | 23418 |
| RRID | AB_2918104 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P82279 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
CRB1, also known as protein crumbs homolog 1, is homologous to Drosophila Crumbs, a protein that is essential for establishing and maintaining apico-basal polarity in epithelia derived from the ectoderm (PMID: 2344615). CRB1 protein is a single transmembrane protein with a large extracellular part, containing three laminin A G domains and 19 epidermal growth-factor-like domains (PMID: 15494026, 18407265, 11734541). The expression of CRB1 is restricted to the retina and brain (PMID: 10508521). Mutations in CRB1 gene cause severe retinal dystrophies, ranging from retinitis pigmentosa (RP) to Leber congenital amaurosis (LCA) (PMID: 11734541).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CRB1 antibody 26598-1-AP | Download protocol |
| IHC protocol for CRB1 antibody 26598-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The CRB1 Polyclonal antibody (catalog number 26598-1-AP) has a total of 1 citation. It appears in the journal Experimental Eye Research (Exp Eye Res), published in 2025. The associated research field is ophthalmology/retinal biology, specifically investigating CRB1 mutations causing structural and molecular defects in patient-derived retinal pigment epithelium cells. The antibody was applied in Western Blot (WB).
| Species | Application | Title |
|---|---|---|
Exp Eye Res CRB1 mutations cause structural and molecular defects in patient-derived retinal pigment epithelium cells |
Reviews
What customers say
The product received a 5-star rating. The reviewer used it at a 1:400 dilution for immunofluorescence on retinal organoids and described the performance as "Excellent!" Overall, the single review reflects strong satisfaction with the antibody's staining quality and reliability in IF applications.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Danyun (Verified Customer) (09-03-2025) | Excellent!
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