Tested Applications
| Positive IHC detected in | mouse lung tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse lung tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
26602-1-AP targets GJA5/Connexin 40 in IHC, IF-P, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag24266 Product name: Recombinant human GJA5 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 233-341 aa of BC013313 Sequence: KIRQRFVKPRQHMAKCQLSGPSVGIVQSCTPPPDFNQCLENGPGGKFFNPFSNNMASQQNTDNLVTEQVRGQEQTPGEGFIQVRYGQKPEVPNGVSPGHRLPHGYHSDK Predict reactive species |
| Full Name | gap junction protein, alpha 5, 40kDa |
| Calculated Molecular Weight | 40 kDa |
| GenBank Accession Number | BC013313 |
| Gene Symbol | GJA5 |
| Gene ID (NCBI) | 2702 |
| RRID | AB_3669552 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P36382 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Connexin 40 (Cx40), also known as Gap junction alpha 5 protein (GJA5), belongs to the connexin family. Connexins are membrane-spanning proteins that assemble to form gap junction channels that facilitate the transfer of ions and small molecules between cells. Four connexins (Cxs) are expressed in the cardiovascular system (Cx37, Cx40, Cx43, and Cx45) and are involved in the development of the cardiovascular system(PMID: 37331352; PMID: 17922338).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for GJA5/Connexin 40 antibody 26602-1-AP | Download protocol |
| IHC protocol for GJA5/Connexin 40 antibody 26602-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The GJA5/Connexin 40 Polyclonal antibody (Cat# 26602-1-AP) has 1 citation. It appears in Cell Signal (IF 4.7), published in 2026. The cited study investigates Smyd1 signaling activation and its role in improving mitochondrial function to reduce diabetes-associated atrial fibrillation via the PGC-1α pathway. The research field encompasses cardiovascular biology, specifically atrial fibrillation pathophysiology, mitochondrial dysfunction, and metabolic signaling in diabetes.
| Species | Application | Title |
|---|---|---|
Cell Signal Smyd1 signaling activation improves mitochondrial function and reduces diabetes-associated atrial fibrillation by enhancing PGC-1α signaling pathway. |
