Tested Applications
| Positive WB detected in | HeLa cells, NIH/3T3 cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | mouse stomach tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
26678-1-AP targets INPPL1 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag24790 Product name: Recombinant human INPPL1 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 74-366 aa of BC140853 Sequence: DGEDFLAVQTSQGVPVRRFQTLGELIGLYAQPNQGLVCALLLPVEGEREPDPPDDRDASDGEDEKPPLPPRSGSTSISAPTGPSSPLPAPETPTAPAAESAPNGLSTVSHDYLKGSYGLDLEAVRGGASHLPHLTRTLATSCRRLHSEVDKVLSGLEILSKVFDQQSSPMVTRLLQQQNLPQTGEQELESLVLKLSVLKDFLSGIQKKALKALQDMSSTAPPAPQPSTRKAKTIPVQAFEVKLDVTLGDLTKIGKSQKFTLSVDVEGGRLVLLRRQRDSQEDWTTFTHDRIRQ Predict reactive species |
| Full Name | inositol polyphosphate phosphatase-like 1 |
| Calculated Molecular Weight | 1258 aa, 139 kDa |
| Observed Molecular Weight | 150 kDa |
| GenBank Accession Number | BC140853 |
| Gene Symbol | INPPL1 |
| Gene ID (NCBI) | 3636 |
| RRID | AB_3085891 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O15357 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for INPPL1 antibody 26678-1-AP | Download protocol |
| IP protocol for INPPL1 antibody 26678-1-AP | Download protocol |
| WB protocol for INPPL1 antibody 26678-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The INPPL1 Polyclonal antibody (Cat# 26678-1-AP) has 1 citation to date. It was cited in Clinical and Translational Medicine (IF 7.9, 2025) in a study on organoid-based drug screening for chemotherapy-resistant oesophageal squamous cell carcinoma. The antibody was used in Western blot (WB) with human samples and RNAi validation. The associated research field is oncology, specifically esophageal cancer and drug resistance.
| Species | Application | Title |
|---|---|---|
Clin Transl Med Organoid-based two-step drug screening for rapid identification of chemotherapy-resistant oesophageal squamous cell carcinoma and alternative therapies.
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