RRP12 Polyclonal antibody

RRP12 Polyclonal Antibody for WB, IF/ICC, ELISA

Cat No. 26849-1-AP

Publications (1)

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Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

KIAA0690, RRP12, RRP12 like protein

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

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Filter: AllWBIF/ICC

WB analysis using 26849-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 26849-1-AP (RRP12 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IF Staining of HeLa using 26849-1-AP
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using RRP12 antibody (26849-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Overview

Tested Applications
Product Information
Publications (1)
Protocols

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MSDS SDS

Tested Applications

Positive WB detected in	HEK-293 cells, HeLa cells, HepG2 cells
Positive IF/ICC detected in	HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:20000-1:100000
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

26849-1-AP targets RRP12 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag25271 Product name: Recombinant human RRP12 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-151 aa of BC012745 Sequence: MGRSGKLPSGVSAKLKRWKKGHSSDSNPAICRHRQAARSRFFSRPSGRSDLTVDAVKLHNELQSGSLRLGKSEAPETPMEEEAELVLTEKSSGTFLSGLSDCTNVTFSKVQRFWESNSAAHKEICAVLAAVTEVIRSQGGKETETEYFAAL Predict reactive species
Full Name	ribosomal RNA processing 12 homolog (S. cerevisiae)
Calculated Molecular Weight	1297 aa, 144 kDa
Observed Molecular Weight	144-150 kDa
GenBank Accession Number	BC012745
Gene Symbol	RRP12
Gene ID (NCBI)	23223
RRID	AB_3669571
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q5JTH9
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
IF protocol for RRP12 antibody 26849-1-AP	Download protocol
WB protocol for RRP12 antibody 26849-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

What published studies show

The RRP12 Polyclonal antibody (Cat# 26849-1-AP) has 1 total citation. It was cited in Cell Reports (Impact Factor 7.7) in a study titled "Nucleoplasmic checkpoint of the 40S ribosomal decoding center maturation" by Benjamin Lau (2026). The research field is ribosome biogenesis and RNA processing, specifically examining the role of RRP12 in 40S ribosomal subunit maturation within the nucleoplasm.