Tested Applications
| Positive WB detected in | A549 cells, HUVEC cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
27124-1-AP targets ADAM15 in WB, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag25947 Product name: Recombinant human ADAM15 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 362-486 aa of BC014566 Sequence: GNSCPCPGPAPAKTCIMEASTDFLPGLNFSNCSRRALEKALLDGMGSCLFERLPSLPPMAAFCGNMFVEPGEQCDCGFLDDCVDPCCDSLTCQLRPGAQCASDGPCCQNCQLRPSGWQCRPTRGD Predict reactive species |
| Full Name | ADAM metallopeptidase domain 15 |
| Calculated Molecular Weight | 93 kDa |
| Observed Molecular Weight | 70 kDa |
| GenBank Accession Number | BC014566 |
| Gene Symbol | ADAM15 |
| Gene ID (NCBI) | 8751 |
| RRID | AB_2923577 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q13444 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
ADAM15, a member of the ADAM (a disintegrin and metalloprotease) family, is a membrane protein containing both protease and adhesion domains and may be involved in tumor invasion and metastasis (PMID:15756594). It is also named as MDC15. ADAM15 is required for the activation of the FAK and Src pathways to generate apoptosis resistance in response to apoptotic signalling or cell stress (PMID: 11741929). ADAM15 is processed during epididymal maturation and acrosome reaction and that it may play a role during sperm-egg binding. Jurkat and testicular cells expresses the precursor ADAM15 of 110 kDa, as well as the ADAM15 processing form, consisting of about 75-85 kDa (PMID:18390692). ADAM15 has some isoforms with the calculated molecular mass of 55~92 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for ADAM15 antibody 27124-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The ADAM15 Polyclonal antibody (Cat# 27124-1-AP) has a total of 1 citation. This citation appears in Research Square (Res Sq), published in June 2025. The cited study, titled "ADAM17 is an HIV-1 Restriction Factor Antagonized by Nef," was conducted using Western Blot (WB) on human samples. The research field is associated with virology and HIV biology, specifically investigating restriction factors and their antagonism by viral proteins.
| Species | Application | Title |
|---|---|---|
