Tested Applications
| Positive WB detected in | HEK-293T cells, mouse brain tissue, HeLa cells, Jurkat cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
27392-1-AP targets OTULIN in WB, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag22302 Product name: Recombinant human FAM105B protein Source: e coli.-derived, PET30a Tag: 6*His Domain: 1-214 aa of BC007706 Sequence: MSQAVGLPPWLQDPELMLLPEKLISKYNWIKQWKLGLKFDGKNEDLVDKIKESLTLLRKKWAGLAEMRTAEARQIACDELFTNEAEEYSLYEAVKFLMLNRAIELYNDKEKGKEVPFFSVLLFARDTSNDPGQLLRNHLNQVGHTGGLEQVEMFLLAYAVRHTIQVYRLSKYNTEEFITVYPTDPPKDWPVVTLIAEDDRHYNIPVRVCEETSL Predict reactive species |
| Full Name | family with sequence similarity 105, member B |
| Calculated Molecular Weight | 352 aa, 40 kDa |
| Observed Molecular Weight | 40-50 kDa |
| GenBank Accession Number | BC007706 |
| Gene Symbol | FAM105B |
| Gene ID (NCBI) | 90268 |
| RRID | AB_3085952 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q96BN8 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Linear ubiquitination is negatively regulated by OTULIN (OTU deubiquitinase with linear linkage specificity, also known as Fam105b or Gumby), a deubiquitinase specifically catalysing the degradation of linear ubiquitin chains. OUb signalling is antagonised by deubiquitinases (DUBs), which cleave the polyUb signal from substrates to terminate signalling. OTU DUB with linear linkage specificity (OTULIN) and CYLD are the two main DUBs that regulate M1-polyUb signalling. OTULIN exclusively cleaves M1 linkages, whereas CYLD cleaves both M1 and K63 linkages. OTULIN binds directly to the LUBAC subunit HOIP and regulates LUBAC signalling, autoubiquitination, and stability.(PMID: 34625557, PMID: 32231246)
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for OTULIN antibody 27392-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The OTULIN Polyclonal antibody (Cat# 27392-1-AP) has 1 total citation. The cited publication appeared in Cellular and Molecular Biology Letters (Impact Factor: 12.2), published in 2026. The research focuses on neonatal lung injury protection by OTULIN under hyperoxia conditions, involving mitochondrial protein OPA1 modulation and its association with the E3 ubiquitin ligase RNF31. The study falls within the fields of pulmonary biology, mitochondrial function, and ubiquitin signaling.
| Species | Application | Title |
|---|---|---|
Cell Mol Biol Lett OTULIN protects hyperoxia-induced neonatal lung injury and modulates mitochondrial protein OPA1 in association with the E3 ubiquitin ligase RNF31. |
