Tested Applications
| Positive WB detected in | NIH/3T3 cells, Raji cells, mouse heart tissue, rat heart tissue |
| Positive IHC detected in | mouse heart tissue, rat heart tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | NIH/3T3 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunohistochemistry (IHC) | IHC : 1:1000-1:4000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
27406-1-AP targets PD-L2/CD273 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag26384 Product name: Recombinant human PDCD1LG2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 20-220 aa of BC074766 Sequence: LFTVTVPKELYIIEHGSNVTLECNFDTGSHVNLGAITASLQKVENDTSPHRERATLLEEQLPLGKASFHIPQVQVRDEGQYQCIIIYGVAWDYKYLTLKVKASYRKINTHILKVPETDEVELTCQATGYPLAEVSWPNVSVPANTSHSRTPEGLYQVTSVLRLKPPPGRNFSCVFWNTHVRELTLASIDLQSQMEPRTHPT Predict reactive species |
| Full Name | programmed cell death 1 ligand 2 |
| Calculated Molecular Weight | 273 aa, 31 kDa |
| Observed Molecular Weight | 45-50 kDa |
| GenBank Accession Number | BC074766 |
| Gene Symbol | PD-L2 |
| Gene ID (NCBI) | 80380 |
| RRID | AB_3085954 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9BQ51 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Programmed cell death 1 ligand 2 (PDCD1LG2), also known as PD-L2, CD273 and B7-DC, is an immune checkpoint receptor ligand which plays a role in negative regulation of the adaptive immune response. PDCD1LG2/PD-L2 protein is expressed mainly by dendritic cells, macrophages, and tumor cells, and down-regulates the effector functions of T cells via PD-1/PD-L2 axis in the tumor microenvironment (PMID: 30275216). The endogenous PDCD1LG2/PD-L2 protein displays a molecular weight of ∼45 kDa(glycosylated form), and a minor band at 35 kDa (non-glycosylated form) in western blot (PMID: 28811964, PMID: 34697216).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for PD-L2/CD273 antibody 27406-1-AP | Download protocol |
| IHC protocol for PD-L2/CD273 antibody 27406-1-AP | Download protocol |
| WB protocol for PD-L2/CD273 antibody 27406-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The PD-L2/CD273 Polyclonal antibody (Cat# 27406-1-AP) has 4 citations published in Biomaterials, International Journal of Oncology, Immunology, and Oncology Research. The associated research fields include cancer immunotherapy, tumor microenvironment remodeling, breast cancer (including triple-negative), esophageal squamous cell carcinoma, autophagy inhibition, and immune evasion mechanisms. Applications cited span WB, IHC, and IF, with mouse as the primary tested species.
| Species | Application | Title |
|---|---|---|
Biomaterials In situ genetically engineering nanosystem for reversing immunosuppression of cancer-associated fibroblasts in breast cancer radiotherapy. | ||
Int J Oncol Discovery of the late autophagy inhibitor FZU‑0045‑053 and its anti‑breast cancer and immunomodulatory effects. | ||
Immunology 5-FU Resistance Facilitates Immune Evasion in Esophageal Squamous Cell Carcinoma Through ADAM10-Mediated PD-L1 Shedding and Tumour Microenvironment Remodelling. | ||
Oncol Res Prognosis and Immunotherapy Effect of Triple-Negative Breast Cancer by Lactylation-Related Genes and Experimental Validation. |
